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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10205 | 3' | -55.7 | NC_002665.1 | + | 26470 | 0.69 | 0.665676 |
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Target: 5'- --cUACccaaGAGGGCCugaauauuCAGGCGCCu -3' miRNA: 3'- auaGUGuag-CUCCCGGuu------GUCCGCGG- -5' |
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| 10205 | 3' | -55.7 | NC_002665.1 | + | 26629 | 1.1 | 0.001654 |
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Target: 5'- cUAUCACAUCGAGGGCCAACAGGCGCCu -3' miRNA: 3'- -AUAGUGUAGCUCCCGGUUGUCCGCGG- -5' |
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| 10205 | 3' | -55.7 | NC_002665.1 | + | 77952 | 0.68 | 0.7381 |
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Target: 5'- --gUACAUCaGAuGGGUCAAagAGGUGCCa -3' miRNA: 3'- auaGUGUAG-CU-CCCGGUUg-UCCGCGG- -5' |
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| 10205 | 3' | -55.7 | NC_002665.1 | + | 79705 | 0.68 | 0.7381 |
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Target: 5'- aUAUCugGggCGGGGGCgG--GGGCGUCa -3' miRNA: 3'- -AUAGugUa-GCUCCCGgUugUCCGCGG- -5' |
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| 10205 | 3' | -55.7 | NC_002665.1 | + | 107643 | 0.66 | 0.840854 |
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Target: 5'- gGUUAUGUUGAGG--UAAUAGGUGCCa -3' miRNA: 3'- aUAGUGUAGCUCCcgGUUGUCCGCGG- -5' |
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| 10205 | 3' | -55.7 | NC_002665.1 | + | 108362 | 0.72 | 0.519397 |
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Target: 5'- aAUC-CAUCgGAGGGCCAucugcauuuACGGG-GCCg -3' miRNA: 3'- aUAGuGUAG-CUCCCGGU---------UGUCCgCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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