miRNA: 3'- aCUUGAUcuUCCCUAUCuaaGUCUaa -5'
miRNA: 3'- acUUGAUCUUCCCUa-UCU------AAGUCUaa -5'
miRNA: 3'- aCUUGAUC--UUCCCU-AUCUaAGUCUaa -5'
miRNA: 3'- aCuuGAUCUUCCCUAUCuaaGUCuaa -5'
miRNA: 3'- -ACUUGAUCUUCCCUAUCUAAGUCUAA- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10206 | 5' | -44.7 | NC_002665.1 | + | 65613 | 0.66 | 0.99992 |
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Target: 5'- aGAACUG--GGGGAUAGg--CAGAg- -3' miRNA: 3'- aCUUGAUcuUCCCUAUCuaaGUCUaa -5' |
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| 10206 | 5' | -44.7 | NC_002665.1 | + | 12163 | 0.67 | 0.999813 |
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Target: 5'- -uGGCUGGAGGGGAacAGAcuuauuUUCAGAa- -3' miRNA: 3'- acUUGAUCUUCCCUa-UCU------AAGUCUaa -5' |
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| 10206 | 5' | -44.7 | NC_002665.1 | + | 9008 | 0.67 | 0.999685 |
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Target: 5'- gGAGCUGGuaAAGGGAcUAGG-UCAGGc- -3' miRNA: 3'- aCUUGAUC--UUCCCU-AUCUaAGUCUaa -5' |
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| 10206 | 5' | -44.7 | NC_002665.1 | + | 61237 | 0.68 | 0.998985 |
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Target: 5'- gGggUUGGAGGGGAUGGugaCAGu-- -3' miRNA: 3'- aCuuGAUCUUCCCUAUCuaaGUCuaa -5' |
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| 10206 | 5' | -44.7 | NC_002665.1 | + | 59430 | 1.06 | 0.034698 |
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Target: 5'- cUGAACUAGAAGGGAUAGAUUCAGAUUc -3' miRNA: 3'- -ACUUGAUCUUCCCUAUCUAAGUCUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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