Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10207 | 5' | -56 | NC_002665.1 | + | 77610 | 0.66 | 0.842446 |
Target: 5'- -uUCAcggGUGUCCCCCCGugGCaCUGGg- -3' miRNA: 3'- ccAGU---UACGGGGGGGUugCG-GGUUac -5' |
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10207 | 5' | -56 | NC_002665.1 | + | 83555 | 0.66 | 0.842446 |
Target: 5'- aGGUCAAUauugGCCCCCCUAagGgGUUCAu-- -3' miRNA: 3'- -CCAGUUA----CGGGGGGGU--UgCGGGUuac -5' |
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10207 | 5' | -56 | NC_002665.1 | + | 40859 | 0.66 | 0.842446 |
Target: 5'- aGGUCAGUuCCCCCCC----UCCAGUa -3' miRNA: 3'- -CCAGUUAcGGGGGGGuugcGGGUUAc -5' |
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10207 | 5' | -56 | NC_002665.1 | + | 59944 | 0.66 | 0.840784 |
Target: 5'- aGGcCGGUGgCCuguCCCCCAugauauggaucuUGCCCAAUGa -3' miRNA: 3'- -CCaGUUAC-GG---GGGGGUu-----------GCGGGUUAC- -5' |
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10207 | 5' | -56 | NC_002665.1 | + | 47642 | 0.69 | 0.700494 |
Target: 5'- uGGUCAGUGCCUCCgCu-CGCuCUAGUu -3' miRNA: 3'- -CCAGUUACGGGGGgGuuGCG-GGUUAc -5' |
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10207 | 5' | -56 | NC_002665.1 | + | 107738 | 0.69 | 0.657943 |
Target: 5'- cGGUCAAggccauuauuuccUGCUUCCCCAAUGUCUucAUGg -3' miRNA: 3'- -CCAGUU-------------ACGGGGGGGUUGCGGGu-UAC- -5' |
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10207 | 5' | -56 | NC_002665.1 | + | 23900 | 0.7 | 0.627588 |
Target: 5'- --cCAGaGCgCCCCCCAACucaCCCAGUGg -3' miRNA: 3'- ccaGUUaCG-GGGGGGUUGc--GGGUUAC- -5' |
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10207 | 5' | -56 | NC_002665.1 | + | 27446 | 0.71 | 0.544582 |
Target: 5'- --cCAGUGCCCCCCUAcugaGCCUAAa- -3' miRNA: 3'- ccaGUUACGGGGGGGUug--CGGGUUac -5' |
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10207 | 5' | -56 | NC_002665.1 | + | 23926 | 0.71 | 0.544582 |
Target: 5'- uGUCuccccaGCCCCCCCAACcCCCAc-- -3' miRNA: 3'- cCAGuua---CGGGGGGGUUGcGGGUuac -5' |
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10207 | 5' | -56 | NC_002665.1 | + | 64646 | 0.73 | 0.437315 |
Target: 5'- uGGggaAAUGCCCCCCCGGgggaauUGUCCAGUa -3' miRNA: 3'- -CCag-UUACGGGGGGGUU------GCGGGUUAc -5' |
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10207 | 5' | -56 | NC_002665.1 | + | 68489 | 1.11 | 0.001413 |
Target: 5'- uGGUCAAUGCCCCCCCAACGCCCAAUGg -3' miRNA: 3'- -CCAGUUACGGGGGGGUUGCGGGUUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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