miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
10208 3' -45.7 NC_002665.1 + 24850 0.66 0.999903
Target:  5'- cGUUggCUUCCGGAGGgGGCGUGuGGu -3'
miRNA:   3'- cCAGa-GAAGGUUUUUgUCGUACuCUg -5'
10208 3' -45.7 NC_002665.1 + 107347 0.66 0.999832
Target:  5'- aGGga-CUUCCAGAGuCAGCAcuguaGGGACa -3'
miRNA:   3'- -CCagaGAAGGUUUUuGUCGUa----CUCUG- -5'
10208 3' -45.7 NC_002665.1 + 52696 0.66 0.999781
Target:  5'- -aUCUCcUUCAAGGGCAGCAccAGAUg -3'
miRNA:   3'- ccAGAGaAGGUUUUUGUCGUacUCUG- -5'
10208 3' -45.7 NC_002665.1 + 108601 0.67 0.999638
Target:  5'- aGGUaCUCUUCUAAGcACuGCA--GGACg -3'
miRNA:   3'- -CCA-GAGAAGGUUUuUGuCGUacUCUG- -5'
10208 3' -45.7 NC_002665.1 + 51485 0.68 0.998879
Target:  5'- uGG-CUUUUCCAGAAAaAGgGUGGGAg -3'
miRNA:   3'- -CCaGAGAAGGUUUUUgUCgUACUCUg -5'
10208 3' -45.7 NC_002665.1 + 85645 0.73 0.952763
Target:  5'- uGGUaaCUggCUAuuGACAGCGUGGGACg -3'
miRNA:   3'- -CCAgaGAa-GGUuuUUGUCGUACUCUG- -5'
10208 3' -45.7 NC_002665.1 + 72427 1.14 0.009936
Target:  5'- aGGUCUCUUCCAAAAACAGCAUGAGACu -3'
miRNA:   3'- -CCAGAGAAGGUUUUUGUCGUACUCUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.