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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10208 | 3' | -45.7 | NC_002665.1 | + | 24850 | 0.66 | 0.999903 |
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Target: 5'- cGUUggCUUCCGGAGGgGGCGUGuGGu -3' miRNA: 3'- cCAGa-GAAGGUUUUUgUCGUACuCUg -5' |
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| 10208 | 3' | -45.7 | NC_002665.1 | + | 107347 | 0.66 | 0.999832 |
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Target: 5'- aGGga-CUUCCAGAGuCAGCAcuguaGGGACa -3' miRNA: 3'- -CCagaGAAGGUUUUuGUCGUa----CUCUG- -5' |
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| 10208 | 3' | -45.7 | NC_002665.1 | + | 52696 | 0.66 | 0.999781 |
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Target: 5'- -aUCUCcUUCAAGGGCAGCAccAGAUg -3' miRNA: 3'- ccAGAGaAGGUUUUUGUCGUacUCUG- -5' |
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| 10208 | 3' | -45.7 | NC_002665.1 | + | 108601 | 0.67 | 0.999638 |
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Target: 5'- aGGUaCUCUUCUAAGcACuGCA--GGACg -3' miRNA: 3'- -CCA-GAGAAGGUUUuUGuCGUacUCUG- -5' |
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| 10208 | 3' | -45.7 | NC_002665.1 | + | 51485 | 0.68 | 0.998879 |
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Target: 5'- uGG-CUUUUCCAGAAAaAGgGUGGGAg -3' miRNA: 3'- -CCaGAGAAGGUUUUUgUCgUACUCUg -5' |
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| 10208 | 3' | -45.7 | NC_002665.1 | + | 85645 | 0.73 | 0.952763 |
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Target: 5'- uGGUaaCUggCUAuuGACAGCGUGGGACg -3' miRNA: 3'- -CCAgaGAa-GGUuuUUGUCGUACUCUG- -5' |
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| 10208 | 3' | -45.7 | NC_002665.1 | + | 72427 | 1.14 | 0.009936 |
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Target: 5'- aGGUCUCUUCCAAAAACAGCAUGAGACu -3' miRNA: 3'- -CCAGAGAAGGUUUUUGUCGUACUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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