miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
10209 5' -51.8 NC_002665.1 + 53083 0.67 0.918243
Target:  5'- uUUAAAUGGGCGa-CCAGUaGGGgGCu -3'
miRNA:   3'- uAAUUUACCCGCagGGUCA-CCUgUGu -5'
10209 5' -51.8 NC_002665.1 + 86269 1.07 0.005669
Target:  5'- gAUUAAAUGGGCGUCCCAGUGGACACAu -3'
miRNA:   3'- -UAAUUUACCCGCAGGGUCACCUGUGU- -5'
10209 5' -51.8 NC_002665.1 + 102622 0.66 0.945241
Target:  5'- ----uAUGGuGUuuagCCCAGUGGAUACAu -3'
miRNA:   3'- uaauuUACC-CGca--GGGUCACCUGUGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.