Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1021 | 3' | -55.2 | NC_000924.1 | + | 50840 | 0.66 | 0.750051 |
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Target: 5'- gCGCCCGGACaaccguuaUuucacggaugagaaaAGCGCCAugGGGAGuGa -3' miRNA: 3'- -GUGGGUCUG--------G---------------UCGCGGUugUUCUCuC- -5' |
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| 1021 | 3' | -55.2 | NC_000924.1 | + | 29432 | 0.67 | 0.648699 |
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Target: 5'- uCAgCCAGGacguCCAGCGUCAGCAAuGAGc- -3' miRNA: 3'- -GUgGGUCU----GGUCGCGGUUGUU-CUCuc -5' |
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| 1021 | 3' | -55.2 | NC_000924.1 | + | 38555 | 0.68 | 0.615636 |
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Target: 5'- aGgCCAGGCUGGCGCaggagAGCGccGGAGAGa -3' miRNA: 3'- gUgGGUCUGGUCGCGg----UUGU--UCUCUC- -5' |
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| 1021 | 3' | -55.2 | NC_000924.1 | + | 51130 | 0.69 | 0.560936 |
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Target: 5'- uGCCCGuGGCaacGCGCCGACGGcAGAGg -3' miRNA: 3'- gUGGGU-CUGgu-CGCGGUUGUUcUCUC- -5' |
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| 1021 | 3' | -55.2 | NC_000924.1 | + | 52558 | 0.69 | 0.560936 |
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Target: 5'- --aCCGGACCAGaaCGCCG-CAAGGGGa -3' miRNA: 3'- gugGGUCUGGUC--GCGGUuGUUCUCUc -5' |
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| 1021 | 3' | -55.2 | NC_000924.1 | + | 46907 | 1.09 | 0.00103 |
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Target: 5'- gCACCCAGACCAGCGCCAACAAGAGAGg -3' miRNA: 3'- -GUGGGUCUGGUCGCGGUUGUUCUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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