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miRNA: 3'- -UGGAUUAGACGGUCAACCUUGGGUCC- -5'
miRNA: 3'- -UGgAUUAGACGGUCa--ACCU---------------UGGGUCC- -5'
miRNA: 3'- -UGGauUAGACGguCA--ACCUUGGGuCC- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 10210 | 3' | -51.8 | NC_002665.1 | + | 97151 | 1.12 | 0.003126 |
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Target: 5'- gACCUAAUCUGCCAGUUGGAACCCAGGg -3' miRNA: 3'- -UGGAUUAGACGGUCAACCUUGGGUCC- -5' |
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| 10210 | 3' | -51.8 | NC_002665.1 | + | 38055 | 0.7 | 0.86038 |
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Target: 5'- cACgUAGUUUGCCAGgagUGGGuggaguguugccgccACCUGGGa -3' miRNA: 3'- -UGgAUUAGACGGUCa--ACCU---------------UGGGUCC- -5' |
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| 10210 | 3' | -51.8 | NC_002665.1 | + | 102226 | 0.68 | 0.901805 |
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Target: 5'- cACUcuGUCUGUguGUgaUGGGACCCuGGc -3' miRNA: 3'- -UGGauUAGACGguCA--ACCUUGGGuCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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