Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10211 | 3' | -45.4 | NC_002665.1 | + | 90028 | 0.66 | 0.999927 |
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Target: 5'- uGUAGcuuuggcugUGGuGGGGAugucuucuuGGUUGGGGAGUg -3' miRNA: 3'- uCAUCa--------ACCuCUCCU---------UUAAUUCCUCA- -5' |
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| 10211 | 3' | -45.4 | NC_002665.1 | + | 83708 | 0.66 | 0.999902 |
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Target: 5'- uGUGGUUGuGGGGGGuccuucuUUGGGGcAGUg -3' miRNA: 3'- uCAUCAAC-CUCUCCuuu----AAUUCC-UCA- -5' |
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| 10211 | 3' | -45.4 | NC_002665.1 | + | 97084 | 0.66 | 0.99987 |
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Target: 5'- uGGU-GUUGGGGGGGuaAAGUUGAG-AGUc -3' miRNA: 3'- -UCAuCAACCUCUCC--UUUAAUUCcUCA- -5' |
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| 10211 | 3' | -45.4 | NC_002665.1 | + | 21132 | 0.67 | 0.999713 |
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Target: 5'- -uUGGggGGuuGGGAGGUUGAGGGa- -3' miRNA: 3'- ucAUCaaCCucUCCUUUAAUUCCUca -5' |
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| 10211 | 3' | -45.4 | NC_002665.1 | + | 63668 | 0.67 | 0.999691 |
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Target: 5'- ---uGUUGGAGAuGGAAGaacaauuuggaucuUUGGGGGGUc -3' miRNA: 3'- ucauCAACCUCU-CCUUU--------------AAUUCCUCA- -5' |
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| 10211 | 3' | -45.4 | NC_002665.1 | + | 23829 | 0.7 | 0.994951 |
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Target: 5'- --gGGUUGGGGucuuGGggGUUugGGGGAGa -3' miRNA: 3'- ucaUCAACCUCu---CCuuUAA--UUCCUCa -5' |
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| 10211 | 3' | -45.4 | NC_002665.1 | + | 90081 | 0.71 | 0.985852 |
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Target: 5'- gAGUuGUUGGAGGGGuAGUgggggcAGGGGUg -3' miRNA: 3'- -UCAuCAACCUCUCCuUUAau----UCCUCA- -5' |
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| 10211 | 3' | -45.4 | NC_002665.1 | + | 89271 | 0.74 | 0.942006 |
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Target: 5'- gGGUAuGUUGGGGGGGA---UGGGGGGc -3' miRNA: 3'- -UCAU-CAACCUCUCCUuuaAUUCCUCa -5' |
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| 10211 | 3' | -45.4 | NC_002665.1 | + | 23770 | 0.74 | 0.936748 |
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Target: 5'- gGGUgAGUUGGGGGgcgcucugguGGggGUUGGGGGGg -3' miRNA: 3'- -UCA-UCAACCUCU----------CCuuUAAUUCCUCa -5' |
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| 10211 | 3' | -45.4 | NC_002665.1 | + | 97264 | 1.1 | 0.019307 |
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Target: 5'- cAGUAGUUGGAGAGGAAAUUAAGGAGUg -3' miRNA: 3'- -UCAUCAACCUCUCCUUUAAUUCCUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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