Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10211 | 5' | -51.2 | NC_002665.1 | + | 89935 | 0.66 | 0.98084 |
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Target: 5'- aGAUaCUCAccccugcccccACU--ACCCCUCCAACAa -3' miRNA: 3'- cCUGaGAGU-----------UGAaaUGGGGGGGUUGU- -5' |
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| 10211 | 5' | -51.2 | NC_002665.1 | + | 68495 | 0.66 | 0.97326 |
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Target: 5'- -aACUauuggUCAAU---GCCCCCCCAACGc -3' miRNA: 3'- ccUGAg----AGUUGaaaUGGGGGGGUUGU- -5' |
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| 10211 | 5' | -51.2 | NC_002665.1 | + | 3351 | 0.66 | 0.97326 |
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Target: 5'- aGGGCaaUUGAU---GCUCCCCCAGCAu -3' miRNA: 3'- -CCUGagAGUUGaaaUGGGGGGGUUGU- -5' |
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| 10211 | 5' | -51.2 | NC_002665.1 | + | 79494 | 0.66 | 0.97326 |
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Target: 5'- aGGuACUgUCAAaag-AUCCaCCCCAACAa -3' miRNA: 3'- -CC-UGAgAGUUgaaaUGGG-GGGGUUGU- -5' |
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| 10211 | 5' | -51.2 | NC_002665.1 | + | 29713 | 0.67 | 0.963716 |
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Target: 5'- aGACUCccCAGau---CCCCCCUAACAu -3' miRNA: 3'- cCUGAGa-GUUgaaauGGGGGGGUUGU- -5' |
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| 10211 | 5' | -51.2 | NC_002665.1 | + | 79367 | 0.67 | 0.956165 |
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Target: 5'- uGGAuCUUUUGACagUACCUCCCCcuuAACAa -3' miRNA: 3'- -CCU-GAGAGUUGaaAUGGGGGGG---UUGU- -5' |
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| 10211 | 5' | -51.2 | NC_002665.1 | + | 42480 | 0.71 | 0.815208 |
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Target: 5'- gGGGCUCuUCAAC---ACCCCCCUguguuuaaGACAu -3' miRNA: 3'- -CCUGAG-AGUUGaaaUGGGGGGG--------UUGU- -5' |
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| 10211 | 5' | -51.2 | NC_002665.1 | + | 81299 | 0.72 | 0.767315 |
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Target: 5'- -uGCUUUCuuuUUUUAUCCCCCCAACu -3' miRNA: 3'- ccUGAGAGuu-GAAAUGGGGGGGUUGu -5' |
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| 10211 | 5' | -51.2 | NC_002665.1 | + | 89129 | 0.75 | 0.615385 |
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Target: 5'- aGGACUC-CAAgccccccauCCCCCCCAACAu -3' miRNA: 3'- -CCUGAGaGUUgaaau----GGGGGGGUUGU- -5' |
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| 10211 | 5' | -51.2 | NC_002665.1 | + | 97221 | 1.11 | 0.004357 |
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Target: 5'- gGGACUCUCAACUUUACCCCCCCAACAc -3' miRNA: 3'- -CCUGAGAGUUGAAAUGGGGGGGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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