Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10212 | 5' | -51.4 | NC_002665.1 | + | 98248 | 0.89 | 0.090453 |
Target: 5'- gCUCACCUGUGGGCAA-GGuAACAGGUAg -3' miRNA: 3'- -GAGUGGACACCUGUUaCC-UUGUCCAU- -5' |
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10212 | 5' | -51.4 | NC_002665.1 | + | 98065 | 0.91 | 0.076261 |
Target: 5'- gCUCACCUGUGGACAA-GGuAACAGGUAg -3' miRNA: 3'- -GAGUGGACACCUGUUaCC-UUGUCCAU- -5' |
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10212 | 5' | -51.4 | NC_002665.1 | + | 15045 | 0.69 | 0.897664 |
Target: 5'- cCUCGggUGUGGAU--UGGAACAGGa- -3' miRNA: 3'- -GAGUggACACCUGuuACCUUGUCCau -5' |
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10212 | 5' | -51.4 | NC_002665.1 | + | 1403 | 0.66 | 0.957612 |
Target: 5'- -aCACCUaUGGACAGUu--ACAGGUGg -3' miRNA: 3'- gaGUGGAcACCUGUUAccuUGUCCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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