Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10214 | 5' | -58.8 | NC_002665.1 | + | 102380 | 1.12 | 0.000762 |
Target: 5'- uGGUGUUCACGGGCUCGGCCAGGGUCCc -3' miRNA: 3'- -CCACAAGUGCCCGAGCCGGUCCCAGG- -5' |
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10214 | 5' | -58.8 | NC_002665.1 | + | 28640 | 0.67 | 0.660881 |
Target: 5'- uGGUGgUCugGGGUauaacaagUGuGCCGGGG-CCg -3' miRNA: 3'- -CCACaAGugCCCGa-------GC-CGGUCCCaGG- -5' |
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10214 | 5' | -58.8 | NC_002665.1 | + | 100422 | 0.71 | 0.472806 |
Target: 5'- -cUGUUUGCuGGGC-CGGCgagugUAGGGUCCg -3' miRNA: 3'- ccACAAGUG-CCCGaGCCG-----GUCCCAGG- -5' |
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10214 | 5' | -58.8 | NC_002665.1 | + | 31028 | 0.67 | 0.691097 |
Target: 5'- aGGUuaaUACGGGCacuuUUGGCUuuuugAGGGUCCu -3' miRNA: 3'- -CCAcaaGUGCCCG----AGCCGG-----UCCCAGG- -5' |
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10214 | 5' | -58.8 | NC_002665.1 | + | 104907 | 0.67 | 0.710024 |
Target: 5'- -cUGUUCACGGGUgggggacUCGGUgGuGGUCUu -3' miRNA: 3'- ccACAAGUGCCCG-------AGCCGgUcCCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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