miRNA: 3'- -ACUGuAGAGGgaGCACC-AUGACGGUG- -5'
miRNA: 3'- -ACUGUAG-AGGGAgcACCA----UGACGGug -5'
miRNA: 3'- acUGUAGAGGGagcACCa----UGACGGUG- -5'
miRNA: 3'- -ACUGUagaGGG----------AGCACCAuGACGGUG- -5'
miRNA: 3'- -ACUGUAGAGGGAGCACCAUGACGGUG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10217 | 3' | -54.7 | NC_002665.1 | + | 8015 | 0.67 | 0.847712 |
|
Target: 5'- gUGACuUCUUUgaUGUGGaUGCUGCCAUc -3' miRNA: 3'- -ACUGuAGAGGgaGCACC-AUGACGGUG- -5' |
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| 10217 | 3' | -54.7 | NC_002665.1 | + | 12025 | 0.68 | 0.803855 |
|
Target: 5'- cUGAUAUCaUCCCUauUGGUaacaGCUGCCu- -3' miRNA: 3'- -ACUGUAG-AGGGAgcACCA----UGACGGug -5' |
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| 10217 | 3' | -54.7 | NC_002665.1 | + | 13143 | 0.67 | 0.821964 |
|
Target: 5'- -uGCAUCUCCCa--UGGcuuucACUGCCACa -3' miRNA: 3'- acUGUAGAGGGagcACCa----UGACGGUG- -5' |
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| 10217 | 3' | -54.7 | NC_002665.1 | + | 52215 | 0.68 | 0.794545 |
|
Target: 5'- gUGACAgcaCCCaaacauuuaaUUGUGGUuCUGCCACa -3' miRNA: 3'- -ACUGUagaGGG----------AGCACCAuGACGGUG- -5' |
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| 10217 | 3' | -54.7 | NC_002665.1 | + | 108439 | 1.09 | 0.002246 |
|
Target: 5'- cUGACAUCUCCCUCGUGGUACUGCCACu -3' miRNA: 3'- -ACUGUAGAGGGAGCACCAUGACGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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