Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10217 | 5' | -50 | NC_002665.1 | + | 1398 | 0.66 | 0.99153 |
Target: 5'- -uAUGGUuugugGGcCAUG-CUGGGGUGUGAa -3' miRNA: 3'- gcUACCG-----UCaGUACaGACUCUACACU- -5' |
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10217 | 5' | -50 | NC_002665.1 | + | 100861 | 0.67 | 0.979236 |
Target: 5'- uGGUGGCAag-AUGUCUcccaucugGGGAUGUGAu -3' miRNA: 3'- gCUACCGUcagUACAGA--------CUCUACACU- -5' |
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10217 | 5' | -50 | NC_002665.1 | + | 33085 | 0.69 | 0.943741 |
Target: 5'- gGAUGGCAcauUCGUGUUUGcuuuuGGUGUGGc -3' miRNA: 3'- gCUACCGUc--AGUACAGACu----CUACACU- -5' |
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10217 | 5' | -50 | NC_002665.1 | + | 108400 | 1.1 | 0.006455 |
Target: 5'- gCGAUGGCAGUCAUGUCUGAGAUGUGAu -3' miRNA: 3'- -GCUACCGUCAGUACAGACUCUACACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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