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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 10218 | 5' | -53.8 | NC_002665.1 | + | 75177 | 0.66 | 0.885521 |
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Target: 5'- ---uGGACAAAuguGUUuCCCaGGGAGGUg -3' miRNA: 3'- auuuCCUGUUUu--CGAcGGG-CCCUCCA- -5' |
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| 10218 | 5' | -53.8 | NC_002665.1 | + | 14954 | 0.66 | 0.903635 |
|
Target: 5'- -cAAGGACAGAcuuggaguugacuGCgGCCUGGGAGa- -3' miRNA: 3'- auUUCCUGUUUu------------CGaCGGGCCCUCca -5' |
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| 10218 | 5' | -53.8 | NC_002665.1 | + | 100313 | 0.66 | 0.878099 |
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Target: 5'- gGGAGGACcgu-GCUGCCgCGGGGc-- -3' miRNA: 3'- aUUUCCUGuuuuCGACGG-GCCCUcca -5' |
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| 10218 | 5' | -53.8 | NC_002665.1 | + | 17854 | 0.66 | 0.892693 |
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Target: 5'- ---uGGGCu-AGGCUGUgCUGGGGGGg -3' miRNA: 3'- auuuCCUGuuUUCGACG-GGCCCUCCa -5' |
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| 10218 | 5' | -53.8 | NC_002665.1 | + | 70027 | 0.67 | 0.846023 |
|
Target: 5'- uUAuuGGAUuu-GGCUGCCCuGGAuGGUg -3' miRNA: 3'- -AUuuCCUGuuuUCGACGGGcCCU-CCA- -5' |
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| 10218 | 5' | -53.8 | NC_002665.1 | + | 91262 | 0.67 | 0.843471 |
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Target: 5'- aAAAGGAgAAacAAGCUGUcauaugugcaaccaCUGGGAGGa -3' miRNA: 3'- aUUUCCUgUU--UUCGACG--------------GGCCCUCCa -5' |
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| 10218 | 5' | -53.8 | NC_002665.1 | + | 5574 | 0.67 | 0.854386 |
|
Target: 5'- aGGGGGACcu-GGaccCCCGGGAGGg -3' miRNA: 3'- aUUUCCUGuuuUCgacGGGCCCUCCa -5' |
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| 10218 | 5' | -53.8 | NC_002665.1 | + | 176 | 1.05 | 0.004626 |
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Target: 5'- cUAAAGGACAAAAGCUGCCCGGGAGGUu -3' miRNA: 3'- -AUUUCCUGUUUUCGACGGGCCCUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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