miRNA: 3'- -UCACAGAGAUUUAGUCACAUGGUUCU- -5'
miRNA: 3'- -UCACAGaga-UUUAGUCACAUGgUUCU- -5'
miRNA: 3'- -UCACAGAGAuUUAGucACAUGGUUcu -5'
miRNA: 3'- ucaCAGAGauuuAGUCACAuGGUUCU- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10220 | 5' | -45 | NC_002665.1 | + | 5662 | 1.11 | 0.019307 |
|
Target: 5'- cAGUGUCUCUAAAUCAGUGUACCAAGAc -3' miRNA: 3'- -UCACAGAGAUUUAGUCACAUGGUUCU- -5' |
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| 10220 | 5' | -45 | NC_002665.1 | + | 35819 | 0.7 | 0.994951 |
|
Target: 5'- cAGUGUCacacGAGUCAGUGUACaAGGGa -3' miRNA: 3'- -UCACAGaga-UUUAGUCACAUGgUUCU- -5' |
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| 10220 | 5' | -45 | NC_002665.1 | + | 49560 | 0.68 | 0.999632 |
|
Target: 5'- gAGUGUCUCUuGAUUuuUGUACUAAu- -3' miRNA: 3'- -UCACAGAGAuUUAGucACAUGGUUcu -5' |
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| 10220 | 5' | -45 | NC_002665.1 | + | 87529 | 0.68 | 0.999632 |
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Target: 5'- --aGUCUCagu-UCAGUGU-CCAGGGg -3' miRNA: 3'- ucaCAGAGauuuAGUCACAuGGUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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