Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10221 | 3' | -48.1 | NC_002665.1 | + | 65452 | 0.66 | 0.997922 |
Target: 5'- cCCCUAUCCAugUCCAAuauaUGGCu-- -3' miRNA: 3'- aGGGGUAGGUuaAGGUUug--ACUGuag -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 4743 | 0.66 | 0.997502 |
Target: 5'- gUCCCCAgugUCCAAguugu-GCUGACcUCa -3' miRNA: 3'- -AGGGGU---AGGUUaagguuUGACUGuAG- -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 17370 | 0.66 | 0.997065 |
Target: 5'- aUCCCCGUCCAGgggggguaUGGCAUg -3' miRNA: 3'- -AGGGGUAGGUUaagguuugACUGUAg -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 4437 | 0.66 | 0.997013 |
Target: 5'- cCCCCAcCCAGaUCCAuuGCgaaACAUCa -3' miRNA: 3'- aGGGGUaGGUUaAGGUu-UGac-UGUAG- -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 73619 | 0.66 | 0.997013 |
Target: 5'- cCCCCcUCCAGggCCA---UGACAUg -3' miRNA: 3'- aGGGGuAGGUUaaGGUuugACUGUAg -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 54237 | 0.66 | 0.99696 |
Target: 5'- gUCCCCGUCCAGUgggguucUCUAAguuuucuaucAUUGAUAa- -3' miRNA: 3'- -AGGGGUAGGUUA-------AGGUU----------UGACUGUag -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 39555 | 0.66 | 0.996447 |
Target: 5'- aCCUCcUCUAAUauagCCAAugUGGCAUUu -3' miRNA: 3'- aGGGGuAGGUUAa---GGUUugACUGUAG- -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 88194 | 0.66 | 0.995796 |
Target: 5'- gCCUCAUCCAAUgggCCA--UUGAC-UCu -3' miRNA: 3'- aGGGGUAGGUUAa--GGUuuGACUGuAG- -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 97203 | 0.66 | 0.995796 |
Target: 5'- cCCCCAacaCCAAaacgUCCAcaaccGGCUGACAc- -3' miRNA: 3'- aGGGGUa--GGUUa---AGGU-----UUGACUGUag -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 26067 | 0.67 | 0.995051 |
Target: 5'- aUCCCC-UCCA---CCAAACUGG-GUCu -3' miRNA: 3'- -AGGGGuAGGUuaaGGUUUGACUgUAG- -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 107807 | 0.67 | 0.995051 |
Target: 5'- cUCCUgGUCCAGagUgCAAGCUGACc-- -3' miRNA: 3'- -AGGGgUAGGUUa-AgGUUUGACUGuag -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 89453 | 0.67 | 0.994202 |
Target: 5'- gCCCCAagccUCCAcaUCC-AGCUGAUAc- -3' miRNA: 3'- aGGGGU----AGGUuaAGGuUUGACUGUag -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 73817 | 0.67 | 0.990932 |
Target: 5'- aCCCCAUCCAaguauuuuuacuAUUCUuuGGgUGAUGUCu -3' miRNA: 3'- aGGGGUAGGU------------UAAGGu-UUgACUGUAG- -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 101733 | 0.68 | 0.989422 |
Target: 5'- aUCUCCAUCCAuauaaagaCCAAugcacgcACUGugAUCu -3' miRNA: 3'- -AGGGGUAGGUuaa-----GGUU-------UGACugUAG- -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 37749 | 0.68 | 0.986359 |
Target: 5'- -aCCCAugucagaggUCCAGUacCCAugauACUGGCAUCa -3' miRNA: 3'- agGGGU---------AGGUUAa-GGUu---UGACUGUAG- -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 76395 | 0.69 | 0.972158 |
Target: 5'- uUCCCUGUCUuguacauugaggGAUUUCAgauGAUUGACAUCu -3' miRNA: 3'- -AGGGGUAGG------------UUAAGGU---UUGACUGUAG- -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 12042 | 0.75 | 0.813605 |
Target: 5'- uUCCCCuccagCCAGUggCCGGACUGACu-- -3' miRNA: 3'- -AGGGGua---GGUUAa-GGUUUGACUGuag -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 64090 | 0.77 | 0.689044 |
Target: 5'- uUCCCCAUCCGccUCCAcgccccCUGACAUUu -3' miRNA: 3'- -AGGGGUAGGUuaAGGUuu----GACUGUAG- -5' |
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10221 | 3' | -48.1 | NC_002665.1 | + | 14506 | 1.13 | 0.006568 |
Target: 5'- aUCCCCAUCCAAUUCCAAACUGACAUCa -3' miRNA: 3'- -AGGGGUAGGUUAAGGUUUGACUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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