Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10224 | 5' | -49.1 | NC_002665.1 | + | 97921 | 0.66 | 0.991971 |
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Target: 5'- aAGCaCCUGUCGgugaGGUGACAGGuACa -3' miRNA: 3'- aUUGgGGACAGUaa--UCGUUGUCCuUG- -5' |
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| 10224 | 5' | -49.1 | NC_002665.1 | + | 41833 | 0.67 | 0.984127 |
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Target: 5'- aAGCCUCUGgCAcUGGU-AUAGGAGCa -3' miRNA: 3'- aUUGGGGACaGUaAUCGuUGUCCUUG- -5' |
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| 10224 | 5' | -49.1 | NC_002665.1 | + | 105550 | 0.67 | 0.982025 |
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Target: 5'- gGACaucgCCUGUCuaacacUGGCAACAacGGAACa -3' miRNA: 3'- aUUGg---GGACAGua----AUCGUUGU--CCUUG- -5' |
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| 10224 | 5' | -49.1 | NC_002665.1 | + | 81981 | 0.68 | 0.974468 |
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Target: 5'- gGugCCCUGUUAUgu-CAugGGGGAUa -3' miRNA: 3'- aUugGGGACAGUAaucGUugUCCUUG- -5' |
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| 10224 | 5' | -49.1 | NC_002665.1 | + | 103132 | 0.7 | 0.908768 |
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Target: 5'- gGACaCCUGUUuugucuagcugaGUUAuGCAACAGGGACa -3' miRNA: 3'- aUUGgGGACAG------------UAAU-CGUUGUCCUUG- -5' |
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| 10224 | 5' | -49.1 | NC_002665.1 | + | 85447 | 0.73 | 0.790502 |
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Target: 5'- -uGCCCCUGUCAgggUAuGUuuGACAGGAGu -3' miRNA: 3'- auUGGGGACAGUa--AU-CG--UUGUCCUUg -5' |
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| 10224 | 5' | -49.1 | NC_002665.1 | + | 22023 | 0.76 | 0.639534 |
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Target: 5'- uUAACCUUUGgCAcUGGUAGCAGGAACa -3' miRNA: 3'- -AUUGGGGACaGUaAUCGUUGUCCUUG- -5' |
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| 10224 | 5' | -49.1 | NC_002665.1 | + | 21848 | 0.92 | 0.090448 |
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Target: 5'- uUAACCCCUGgCAcUAGCAACAGGAACa -3' miRNA: 3'- -AUUGGGGACaGUaAUCGUUGUCCUUG- -5' |
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| 10224 | 5' | -49.1 | NC_002665.1 | + | 21893 | 1.1 | 0.006674 |
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Target: 5'- uUAACCCCUGUCAUUAGCAACAGGAACa -3' miRNA: 3'- -AUUGGGGACAGUAAUCGUUGUCCUUG- -5' |
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| 10224 | 5' | -49.1 | NC_002665.1 | + | 21980 | 1.1 | 0.006674 |
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Target: 5'- uUAACCCCUGUCAUUAGCAACAGGAACa -3' miRNA: 3'- -AUUGGGGACAGUAAUCGUUGUCCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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