Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10226 | 5' | -54.3 | NC_002665.1 | + | 39751 | 0.67 | 0.858547 |
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Target: 5'- cUCUgGGACCACC-ACUGGGaAUACa -3' miRNA: 3'- uGGGgUCUGGUGGuUGACCUaUGUGa -5' |
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| 10226 | 5' | -54.3 | NC_002665.1 | + | 4218 | 0.68 | 0.815863 |
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Target: 5'- uCCCCAaaauCCAacuGCUGGAUACACa -3' miRNA: 3'- uGGGGUcu--GGUgguUGACCUAUGUGa -5' |
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| 10226 | 5' | -54.3 | NC_002665.1 | + | 63565 | 0.69 | 0.768674 |
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Target: 5'- aGCuCCCAacguGACuCACCAGCUGGcuccaGCACUa -3' miRNA: 3'- -UG-GGGU----CUG-GUGGUUGACCua---UGUGA- -5' |
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| 10226 | 5' | -54.3 | NC_002665.1 | + | 43318 | 0.69 | 0.768674 |
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Target: 5'- cCCCCAGACCuuCAGauaggGGGUugGCa -3' miRNA: 3'- uGGGGUCUGGugGUUga---CCUAugUGa -5' |
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| 10226 | 5' | -54.3 | NC_002665.1 | + | 19251 | 0.69 | 0.728491 |
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Target: 5'- cGCCCCAGAgUACCccacuAUUGGAcugagGCACa -3' miRNA: 3'- -UGGGGUCUgGUGGu----UGACCUa----UGUGa -5' |
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| 10226 | 5' | -54.3 | NC_002665.1 | + | 9418 | 0.71 | 0.633794 |
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Target: 5'- gACCCCAGACaCAUUGAcCUGGggGCAUUu -3' miRNA: 3'- -UGGGGUCUG-GUGGUU-GACCuaUGUGA- -5' |
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| 10226 | 5' | -54.3 | NC_002665.1 | + | 28518 | 1.08 | 0.003146 |
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Target: 5'- uACCCCAGACCACCAACUGGAUACACUg -3' miRNA: 3'- -UGGGGUCUGGUGGUUGACCUAUGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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