Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10228 | 3' | -59.2 | NC_002665.1 | + | 23919 | 0.67 | 0.636519 |
Target: 5'- ---cCAGC-CCCCCCaacCCCCAcCAGa -3' miRNA: 3'- uguuGUCGaGGGGGGgu-GGGGUuGUC- -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 100212 | 0.69 | 0.514578 |
Target: 5'- aGCGAC--CUCCaaCCCCACCCCAGgAGu -3' miRNA: 3'- -UGUUGucGAGGg-GGGGUGGGGUUgUC- -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 67274 | 0.7 | 0.411881 |
Target: 5'- -uGACAGaUUUCCCCCCACCCUAu--- -3' miRNA: 3'- ugUUGUC-GAGGGGGGGUGGGGUuguc -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 108713 | 0.73 | 0.280696 |
Target: 5'- cACAGCc-CUCgUCCCCCACCCCAcACAGu -3' miRNA: 3'- -UGUUGucGAG-GGGGGGUGGGGU-UGUC- -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 105007 | 0.77 | 0.167055 |
Target: 5'- aGCAAUGGCagacCCCCCUCACCCCAugAa -3' miRNA: 3'- -UGUUGUCGa---GGGGGGGUGGGGUugUc -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 25916 | 0.66 | 0.6879 |
Target: 5'- -gGACgcguGGC-CCCCCCCguACCCCAcACAc -3' miRNA: 3'- ugUUG----UCGaGGGGGGG--UGGGGU-UGUc -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 17270 | 0.66 | 0.6879 |
Target: 5'- cACAACAGC-CaugaCUUCCACCCUggUGGa -3' miRNA: 3'- -UGUUGUCGaGg---GGGGGUGGGGuuGUC- -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 963 | 0.66 | 0.6879 |
Target: 5'- cCAugGGCUCCCUU--GCCCCAAUg- -3' miRNA: 3'- uGUugUCGAGGGGGggUGGGGUUGuc -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 15128 | 0.66 | 0.657141 |
Target: 5'- gACAGuuuGCUCUgagUCCCCGCCCCAaaACAu -3' miRNA: 3'- -UGUUgu-CGAGG---GGGGGUGGGGU--UGUc -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 66088 | 0.67 | 0.635486 |
Target: 5'- uACAGCAGUUUCCucaacugaguCCCCaaacaccacuuuaACCUCAACAGc -3' miRNA: 3'- -UGUUGUCGAGGG----------GGGG-------------UGGGGUUGUC- -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 86451 | 0.68 | 0.554505 |
Target: 5'- aACAAaAGacaCCCCCUUACCCCAAcCAGa -3' miRNA: 3'- -UGUUgUCga-GGGGGGGUGGGGUU-GUC- -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 5097 | 0.7 | 0.447812 |
Target: 5'- ---uCAGUUCCUCauaCCCACCCaCAGCAGa -3' miRNA: 3'- uguuGUCGAGGGG---GGGUGGG-GUUGUC- -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 83745 | 0.7 | 0.438665 |
Target: 5'- -uGAUgaUUCCCUCCCACCCCAAUAu -3' miRNA: 3'- ugUUGucGAGGGGGGGUGGGGUUGUc -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 90131 | 0.7 | 0.411881 |
Target: 5'- --uACAGCcccaUCCCCCCCaaACCCCAAa-- -3' miRNA: 3'- uguUGUCG----AGGGGGGG--UGGGGUUguc -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 17700 | 0.71 | 0.40318 |
Target: 5'- uCAACAGCgCCCCCagaaaccauaCCcCCCCAGCAc -3' miRNA: 3'- uGUUGUCGaGGGGG----------GGuGGGGUUGUc -5' |
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10228 | 3' | -59.2 | NC_002665.1 | + | 80397 | 1.07 | 0.001238 |
Target: 5'- cACAACAGCUCCCCCCCACCCCAACAGc -3' miRNA: 3'- -UGUUGUCGAGGGGGGGUGGGGUUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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