miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
10229 3' -55.3 NC_002665.1 + 108537 0.7 0.66374
Target:  5'- uGGGCCAGg---GACUgugccaacaUGCCCaGGGCg -3'
miRNA:   3'- -CCCGGUUauaaCUGGa--------GCGGGaCCCG- -5'
10229 3' -55.3 NC_002665.1 + 98382 0.78 0.272183
Target:  5'- cGGGCUAugGUUGuCCUCGCCCauggauugagcgGGGCg -3'
miRNA:   3'- -CCCGGUuaUAACuGGAGCGGGa-----------CCCG- -5'
10229 3' -55.3 NC_002665.1 + 83589 0.66 0.862528
Target:  5'- -uGUCAAgg-UGGCCUCuuccggaaaGCCCaGGGCg -3'
miRNA:   3'- ccCGGUUauaACUGGAG---------CGGGaCCCG- -5'
10229 3' -55.3 NC_002665.1 + 83555 0.7 0.684588
Target:  5'- aGGUCAAUAUUGGCC-C-CCCUaagGGGUu -3'
miRNA:   3'- cCCGGUUAUAACUGGaGcGGGA---CCCG- -5'
10229 3' -55.3 NC_002665.1 + 83431 1.13 0.001156
Target:  5'- gGGGCCAAUAUUGACCUCGCCCUGGGCu -3'
miRNA:   3'- -CCCGGUUAUAACUGGAGCGGGACCCG- -5'
10229 3' -55.3 NC_002665.1 + 71557 0.69 0.694949
Target:  5'- uGGGCC-AUAUagcUGAgCUgGUCCcGGGCu -3'
miRNA:   3'- -CCCGGuUAUA---ACUgGAgCGGGaCCCG- -5'
10229 3' -55.3 NC_002665.1 + 46339 0.67 0.812146
Target:  5'- -uGCCAggaGUGUUaAUCUUGCUCUGGGUu -3'
miRNA:   3'- ccCGGU---UAUAAcUGGAGCGGGACCCG- -5'
10229 3' -55.3 NC_002665.1 + 25281 0.66 0.884869
Target:  5'- -aGCCucuuUAUaGACCcCGCCCUGGuGUc -3'
miRNA:   3'- ccCGGuu--AUAaCUGGaGCGGGACC-CG- -5'
10229 3' -55.3 NC_002665.1 + 24965 0.69 0.725661
Target:  5'- -aGCCAacgagAUGUuccaacacgUGGCCcuaugUGCCCUGGGCa -3'
miRNA:   3'- ccCGGU-----UAUA---------ACUGGa----GCGGGACCCG- -5'
10229 3' -55.3 NC_002665.1 + 24828 0.67 0.803099
Target:  5'- aGGGCCAcGUGUUGGaacaUCUCGUuggcuuCCggaggGGGCg -3'
miRNA:   3'- -CCCGGU-UAUAACU----GGAGCG------GGa----CCCG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.