Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10229 | 3' | -55.3 | NC_002665.1 | + | 98382 | 0.78 | 0.272183 |
Target: 5'- cGGGCUAugGUUGuCCUCGCCCauggauugagcgGGGCg -3' miRNA: 3'- -CCCGGUuaUAACuGGAGCGGGa-----------CCCG- -5' |
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10229 | 3' | -55.3 | NC_002665.1 | + | 83555 | 0.7 | 0.684588 |
Target: 5'- aGGUCAAUAUUGGCC-C-CCCUaagGGGUu -3' miRNA: 3'- cCCGGUUAUAACUGGaGcGGGA---CCCG- -5' |
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10229 | 3' | -55.3 | NC_002665.1 | + | 71557 | 0.69 | 0.694949 |
Target: 5'- uGGGCC-AUAUagcUGAgCUgGUCCcGGGCu -3' miRNA: 3'- -CCCGGuUAUA---ACUgGAgCGGGaCCCG- -5' |
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10229 | 3' | -55.3 | NC_002665.1 | + | 24965 | 0.69 | 0.725661 |
Target: 5'- -aGCCAacgagAUGUuccaacacgUGGCCcuaugUGCCCUGGGCa -3' miRNA: 3'- ccCGGU-----UAUA---------ACUGGa----GCGGGACCCG- -5' |
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10229 | 3' | -55.3 | NC_002665.1 | + | 83589 | 0.66 | 0.862528 |
Target: 5'- -uGUCAAgg-UGGCCUCuuccggaaaGCCCaGGGCg -3' miRNA: 3'- ccCGGUUauaACUGGAG---------CGGGaCCCG- -5' |
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10229 | 3' | -55.3 | NC_002665.1 | + | 25281 | 0.66 | 0.884869 |
Target: 5'- -aGCCucuuUAUaGACCcCGCCCUGGuGUc -3' miRNA: 3'- ccCGGuu--AUAaCUGGaGCGGGACC-CG- -5' |
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10229 | 3' | -55.3 | NC_002665.1 | + | 83431 | 1.13 | 0.001156 |
Target: 5'- gGGGCCAAUAUUGACCUCGCCCUGGGCu -3' miRNA: 3'- -CCCGGUUAUAACUGGAGCGGGACCCG- -5' |
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10229 | 3' | -55.3 | NC_002665.1 | + | 108537 | 0.7 | 0.66374 |
Target: 5'- uGGGCCAGg---GACUgugccaacaUGCCCaGGGCg -3' miRNA: 3'- -CCCGGUUauaaCUGGa--------GCGGGaCCCG- -5' |
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10229 | 3' | -55.3 | NC_002665.1 | + | 24828 | 0.67 | 0.803099 |
Target: 5'- aGGGCCAcGUGUUGGaacaUCUCGUuggcuuCCggaggGGGCg -3' miRNA: 3'- -CCCGGU-UAUAACU----GGAGCG------GGa----CCCG- -5' |
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10229 | 3' | -55.3 | NC_002665.1 | + | 46339 | 0.67 | 0.812146 |
Target: 5'- -uGCCAggaGUGUUaAUCUUGCUCUGGGUu -3' miRNA: 3'- ccCGGU---UAUAAcUGGAGCGGGACCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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