miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
10229 5' -55.3 NC_002665.1 + 24263 0.66 0.881404
Target:  5'- gGGGCCACuCUUucCAGGGGaaGAAuCCAc -3'
miRNA:   3'- -UCCGGUG-GAAcuGUCUCCagCUU-GGU- -5'
10229 5' -55.3 NC_002665.1 + 68464 0.67 0.805438
Target:  5'- uGGGCCACCUaaacuuuaugggGAaaaGGGGGUUGAgguuucacaACCAa -3'
miRNA:   3'- -UCCGGUGGAa-----------CUg--UCUCCAGCU---------UGGU- -5'
10229 5' -55.3 NC_002665.1 + 102925 0.69 0.688539
Target:  5'- uGGUgGCUcaacaGGCAGGGGUUGGGCCAc -3'
miRNA:   3'- uCCGgUGGaa---CUGUCUCCAGCUUGGU- -5'
10229 5' -55.3 NC_002665.1 + 83467 1.09 0.00229
Target:  5'- gAGGCCACCUUGACAGAGGUCGAACCAu -3'
miRNA:   3'- -UCCGGUGGAACUGUCUCCAGCUUGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.