Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1023 | 5' | -47.7 | NC_000924.1 | + | 57701 | 0.66 | 0.981461 |
Target: 5'- aUACCGGCA--AUCAG-AAUUGCGg- -3' miRNA: 3'- aAUGGCUGUuaUAGUCaUUGGCGCaa -5' |
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1023 | 5' | -47.7 | NC_000924.1 | + | 17011 | 0.67 | 0.97331 |
Target: 5'- -cACCGuacaGCAAUAUCAG--GCCGUGg- -3' miRNA: 3'- aaUGGC----UGUUAUAGUCauUGGCGCaa -5' |
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1023 | 5' | -47.7 | NC_000924.1 | + | 31516 | 0.67 | 0.97009 |
Target: 5'- gUUAUgGAUAGUcAUCGGcUGACCGCGa- -3' miRNA: 3'- -AAUGgCUGUUA-UAGUC-AUUGGCGCaa -5' |
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1023 | 5' | -47.7 | NC_000924.1 | + | 24378 | 0.67 | 0.966601 |
Target: 5'- -cAUCGACAGUAUCAGagGugCGCc-- -3' miRNA: 3'- aaUGGCUGUUAUAGUCa-UugGCGcaa -5' |
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1023 | 5' | -47.7 | NC_000924.1 | + | 16056 | 0.67 | 0.958782 |
Target: 5'- cUGCCGcACGAugcUAUCcgcGUGACCGUGUUc -3' miRNA: 3'- aAUGGC-UGUU---AUAGu--CAUUGGCGCAA- -5' |
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1023 | 5' | -47.7 | NC_000924.1 | + | 31970 | 0.69 | 0.921915 |
Target: 5'- -gGCUGGCcu--UCAGUAACCGUGg- -3' miRNA: 3'- aaUGGCUGuuauAGUCAUUGGCGCaa -5' |
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1023 | 5' | -47.7 | NC_000924.1 | + | 57335 | 0.71 | 0.861232 |
Target: 5'- aUUGCCGugGA--UCGGUuuAACCGCGa- -3' miRNA: 3'- -AAUGGCugUUauAGUCA--UUGGCGCaa -5' |
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1023 | 5' | -47.7 | NC_000924.1 | + | 49486 | 1.03 | 0.014326 |
Target: 5'- uUUACCGACAAUAUCAGUAACCGCGUUu -3' miRNA: 3'- -AAUGGCUGUUAUAGUCAUUGGCGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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