miRNA display CGI


Results 1 - 3 of 3 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
10230 3' -53 NC_002665.1 + 86132 1.07 0.004362
Target:  5'- cCAUGUGUCCACUGGGACGCCCAUUUAa -3'
miRNA:   3'- -GUACACAGGUGACCCUGCGGGUAAAU- -5'
10230 3' -53 NC_002665.1 + 3586 0.66 0.924655
Target:  5'- aAUGUGUCCACaUGGG-C-CCUAUa-- -3'
miRNA:   3'- gUACACAGGUG-ACCCuGcGGGUAaau -5'
10230 3' -53 NC_002665.1 + 83679 0.66 0.918792
Target:  5'- --aGUGUCUAUaucuuUGGGAUGUCCAc--- -3'
miRNA:   3'- guaCACAGGUG-----ACCCUGCGGGUaaau -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.