Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
10231 | 5' | -47.6 | NC_002665.1 | + | 65165 | 0.66 | 0.999154 |
Target: 5'- aCCUgGAUgGgcCcUCCAAUGGGUCAuuGGUa -3' miRNA: 3'- -GGAgUUAgU--GuAGGUUACCUAGU--CCG- -5' |
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10231 | 5' | -47.6 | NC_002665.1 | + | 42345 | 0.66 | 0.999154 |
Target: 5'- cCUUCA---AUAUCCucuacGUGGAUgAGGCa -3' miRNA: 3'- -GGAGUuagUGUAGGu----UACCUAgUCCG- -5' |
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10231 | 5' | -47.6 | NC_002665.1 | + | 97091 | 0.66 | 0.999154 |
Target: 5'- aCCaUCGAUCugAUCCcuaGAUGGGUau-GCa -3' miRNA: 3'- -GG-AGUUAGugUAGG---UUACCUAgucCG- -5' |
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10231 | 5' | -47.6 | NC_002665.1 | + | 29456 | 0.67 | 0.998115 |
Target: 5'- ------aCACGUCCAGUaGAUUGGGCu -3' miRNA: 3'- ggaguuaGUGUAGGUUAcCUAGUCCG- -5' |
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10231 | 5' | -47.6 | NC_002665.1 | + | 11856 | 0.67 | 0.997731 |
Target: 5'- aCCUCAGUgACAguuuaCAgaggGUGGuccCAGGCa -3' miRNA: 3'- -GGAGUUAgUGUag---GU----UACCua-GUCCG- -5' |
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10231 | 5' | -47.6 | NC_002665.1 | + | 4587 | 0.68 | 0.994697 |
Target: 5'- uUCUCAcAUCACuuugauguuUCgCAAUGGAUCuGGGUg -3' miRNA: 3'- -GGAGU-UAGUGu--------AG-GUUACCUAG-UCCG- -5' |
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10231 | 5' | -47.6 | NC_002665.1 | + | 55089 | 0.68 | 0.992587 |
Target: 5'- uCC-CAGU-ACGUCCccccauucagGGAUCAGGCu -3' miRNA: 3'- -GGaGUUAgUGUAGGuua-------CCUAGUCCG- -5' |
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10231 | 5' | -47.6 | NC_002665.1 | + | 68408 | 0.71 | 0.960858 |
Target: 5'- uCCUgGGUCACAUCCAucuugAUGGcAUU-GGCc -3' miRNA: 3'- -GGAgUUAGUGUAGGU-----UACC-UAGuCCG- -5' |
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10231 | 5' | -47.6 | NC_002665.1 | + | 87966 | 0.72 | 0.95272 |
Target: 5'- aCCUCG---GCGUCUggUGGGUaUAGGCc -3' miRNA: 3'- -GGAGUuagUGUAGGuuACCUA-GUCCG- -5' |
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10231 | 5' | -47.6 | NC_002665.1 | + | 59226 | 0.72 | 0.948253 |
Target: 5'- uCCUCAAagccggGCGUCUugGGUGGGUCAGGa -3' miRNA: 3'- -GGAGUUag----UGUAGG--UUACCUAGUCCg -5' |
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10231 | 5' | -47.6 | NC_002665.1 | + | 67884 | 0.75 | 0.830924 |
Target: 5'- -aUCAAgcccaGCAUCCAAUGGAUauugGGGCa -3' miRNA: 3'- ggAGUUag---UGUAGGUUACCUAg---UCCG- -5' |
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10231 | 5' | -47.6 | NC_002665.1 | + | 87864 | 1.15 | 0.005896 |
Target: 5'- uCCUCAAUCACAUCCAAUGGAUCAGGCu -3' miRNA: 3'- -GGAGUUAGUGUAGGUUACCUAGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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