Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10232 | 5' | -55.8 | NC_002665.1 | + | 26617 | 0.66 | 0.847777 |
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Target: 5'- gGGCCAA-CAGGcGCCUGaauAUUcagGCCCu -3' miRNA: 3'- -UCGGUUaGUCCcCGGACc--UAAa--CGGG- -5' |
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| 10232 | 5' | -55.8 | NC_002665.1 | + | 52766 | 0.66 | 0.813226 |
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Target: 5'- gGGCCccaaacUCuguGGGGCCUGG---UGCUCc -3' miRNA: 3'- -UCGGuu----AGu--CCCCGGACCuaaACGGG- -5' |
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| 10232 | 5' | -55.8 | NC_002665.1 | + | 91464 | 0.67 | 0.775869 |
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Target: 5'- uGCCA--CAGGGGaCUGuAUUUGCUCu -3' miRNA: 3'- uCGGUuaGUCCCCgGACcUAAACGGG- -5' |
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| 10232 | 5' | -55.8 | NC_002665.1 | + | 59940 | 0.67 | 0.763225 |
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Target: 5'- uGCCAggCcGGuGGCCugucccccaugauaUGGAUcUUGCCCa -3' miRNA: 3'- uCGGUuaGuCC-CCGG--------------ACCUA-AACGGG- -5' |
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| 10232 | 5' | -55.8 | NC_002665.1 | + | 58788 | 0.68 | 0.726163 |
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Target: 5'- uGUC-AUCAGGGG-CUGGGUUaacauggcaUGCCUc -3' miRNA: 3'- uCGGuUAGUCCCCgGACCUAA---------ACGGG- -5' |
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| 10232 | 5' | -55.8 | NC_002665.1 | + | 10529 | 0.68 | 0.726163 |
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Target: 5'- --aCAGUCcugcuuGaGGGCCUGGAUaUUGUCCa -3' miRNA: 3'- ucgGUUAGu-----C-CCCGGACCUA-AACGGG- -5' |
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| 10232 | 5' | -55.8 | NC_002665.1 | + | 89167 | 0.68 | 0.695217 |
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Target: 5'- gGGCCAc---GGGGUCUGG--UUGUCCa -3' miRNA: 3'- -UCGGUuaguCCCCGGACCuaAACGGG- -5' |
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| 10232 | 5' | -55.8 | NC_002665.1 | + | 21283 | 0.77 | 0.264714 |
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Target: 5'- uAGCCAAUCAGGuGUUUGGAUUUgguuguccugGCCCu -3' miRNA: 3'- -UCGGUUAGUCCcCGGACCUAAA----------CGGG- -5' |
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| 10232 | 5' | -55.8 | NC_002665.1 | + | 97595 | 0.88 | 0.051814 |
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Target: 5'- -aCCAAUCAGGGGCCUGGAUUcgauaggccggGCCCu -3' miRNA: 3'- ucGGUUAGUCCCCGGACCUAAa----------CGGG- -5' |
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| 10232 | 5' | -55.8 | NC_002665.1 | + | 97498 | 1.12 | 0.001034 |
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Target: 5'- aAGCCAAUCAGGGGCCUGGAUUUGCCCa -3' miRNA: 3'- -UCGGUUAGUCCCCGGACCUAAACGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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