Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1024 | 3' | -56.2 | NC_000924.1 | + | 56220 | 0.66 | 0.621321 |
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Target: 5'- cGCCGCCugcUGGCGUg-ACAaaCCGGCAu -3' miRNA: 3'- -CGGCGGu--ACCGUAagUGUc-GGUCGUu -5' |
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| 1024 | 3' | -56.2 | NC_000924.1 | + | 47597 | 0.67 | 0.588012 |
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Target: 5'- cGCCGCCAUcuGUuuGUUCGCAGCCu-CAAu -3' miRNA: 3'- -CGGCGGUAc-CG--UAAGUGUCGGucGUU- -5' |
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| 1024 | 3' | -56.2 | NC_000924.1 | + | 4197 | 0.67 | 0.588012 |
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Target: 5'- aGCgCGCCGcUGGCAUUCGCAucaaaagagGUgAGUAAu -3' miRNA: 3'- -CG-GCGGU-ACCGUAAGUGU---------CGgUCGUU- -5' |
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| 1024 | 3' | -56.2 | NC_000924.1 | + | 16536 | 0.68 | 0.490985 |
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Target: 5'- aGgUGCUAUGGCAUggUCaACGGCUGGCGc -3' miRNA: 3'- -CgGCGGUACCGUA--AG-UGUCGGUCGUu -5' |
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| 1024 | 3' | -56.2 | NC_000924.1 | + | 4070 | 0.68 | 0.490985 |
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Target: 5'- cGCCGCCAUaccGGUAUU-GCAGCggcacgacgagCAGCAAa -3' miRNA: 3'- -CGGCGGUA---CCGUAAgUGUCG-----------GUCGUU- -5' |
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| 1024 | 3' | -56.2 | NC_000924.1 | + | 7256 | 0.69 | 0.450235 |
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Target: 5'- uUCGCCAgacGGCAUUUAaaggugauGCCAGCGAu -3' miRNA: 3'- cGGCGGUa--CCGUAAGUgu------CGGUCGUU- -5' |
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| 1024 | 3' | -56.2 | NC_000924.1 | + | 33166 | 0.69 | 0.44034 |
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Target: 5'- cGCCGCCAgGGCAcaUGCAGCU-GCAc -3' miRNA: 3'- -CGGCGGUaCCGUaaGUGUCGGuCGUu -5' |
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| 1024 | 3' | -56.2 | NC_000924.1 | + | 36805 | 0.8 | 0.082583 |
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Target: 5'- gGCUGCCGUGGCAUUCAgCcGCCAGUc- -3' miRNA: 3'- -CGGCGGUACCGUAAGU-GuCGGUCGuu -5' |
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| 1024 | 3' | -56.2 | NC_000924.1 | + | 50091 | 1.08 | 0.000839 |
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Target: 5'- uGCCGCCAUGGCAUUCACAGCCAGCAAu -3' miRNA: 3'- -CGGCGGUACCGUAAGUGUCGGUCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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