miRNA: 3'- cUUUAGUUUACGGGGGauuuuuuauc -5'
miRNA: 3'- -CUUUAGUUUacgGGGGA----------UUUUUUAUC- -5'
miRNA: 3'- cUUUAG-UUUACGGGGGAu-UUUUUAUc -5'
miRNA: 3'- cuuuaGUUUACGGGGGAUUUUUUauc -5'
miRNA: 3'- -CUUUAGUUUACGGGGGAUUUUUUAUC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10240 | 5' | -39.8 | NC_002671.1 | + | 7848 | 0.66 | 0.998759 |
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Target: 5'- cAAAUUAAAUGCCCCUn--------- -3' miRNA: 3'- cUUUAGUUUACGGGGGauuuuuuauc -5' |
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| 10240 | 5' | -39.8 | NC_002671.1 | + | 6659 | 0.68 | 0.989974 |
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Target: 5'- aGAAAUCAGAa--CCCCUuucaaguugaGAGGAGUAGa -3' miRNA: 3'- -CUUUAGUUUacgGGGGA----------UUUUUUAUC- -5' |
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| 10240 | 5' | -39.8 | NC_002671.1 | + | 7864 | 0.73 | 0.877235 |
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Target: 5'- aAAAUUaAAAUGCCCCCUuuGGAAAUAc -3' miRNA: 3'- cUUUAG-UUUACGGGGGAu-UUUUUAUc -5' |
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| 10240 | 5' | -39.8 | NC_002671.1 | + | 7960 | 0.8 | 0.520771 |
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Target: 5'- ----aUAAAUGCCCCCUGAAAAGc-- -3' miRNA: 3'- cuuuaGUUUACGGGGGAUUUUUUauc -5' |
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| 10240 | 5' | -39.8 | NC_002671.1 | + | 7958 | 1.12 | 0.006327 |
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Target: 5'- uGAAAUCAAAUGCCCCCUAAAAAAUAGa -3' miRNA: 3'- -CUUUAGUUUACGGGGGAUUUUUUAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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