Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10241 | 5' | -48.7 | NC_002686.1 | + | 108310 | 0.66 | 0.998539 |
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Target: 5'- aUGCGuu--GAgACGCUU-CGUUGGGa -3' miRNA: 3'- cACGCcuauCUgUGCGAAuGCAAUCC- -5' |
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| 10241 | 5' | -48.7 | NC_002686.1 | + | 119845 | 0.66 | 0.998539 |
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Target: 5'- aUGCGuu--GAgACGCUU-CGUUGGGa -3' miRNA: 3'- cACGCcuauCUgUGCGAAuGCAAUCC- -5' |
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| 10241 | 5' | -48.7 | NC_002686.1 | + | 116391 | 0.66 | 0.997837 |
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Target: 5'- aUGCGGGUAuaACAgggggguuugcagUGCUUGCGUUGGu -3' miRNA: 3'- cACGCCUAUc-UGU-------------GCGAAUGCAAUCc -5' |
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| 10241 | 5' | -48.7 | NC_002686.1 | + | 122049 | 0.67 | 0.995784 |
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Target: 5'- -aGCGGAgUGGACGC-CUUACGgagacGGu -3' miRNA: 3'- caCGCCU-AUCUGUGcGAAUGCaau--CC- -5' |
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| 10241 | 5' | -48.7 | NC_002686.1 | + | 106106 | 0.67 | 0.995784 |
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Target: 5'- -aGCGGAgUGGACGC-CUUACGgagacGGu -3' miRNA: 3'- caCGCCU-AUCUGUGcGAAUGCaau--CC- -5' |
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| 10241 | 5' | -48.7 | NC_002686.1 | + | 123811 | 0.68 | 0.992247 |
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Target: 5'- -cGCGGGUGG-CugGCggACG--AGGg -3' miRNA: 3'- caCGCCUAUCuGugCGaaUGCaaUCC- -5' |
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| 10241 | 5' | -48.7 | NC_002686.1 | + | 104343 | 0.68 | 0.992247 |
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Target: 5'- -cGCGGGUGG-CugGCggACG--AGGg -3' miRNA: 3'- caCGCCUAUCuGugCGaaUGCaaUCC- -5' |
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| 10241 | 5' | -48.7 | NC_002686.1 | + | 53795 | 0.72 | 0.920993 |
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Target: 5'- -cGCG--UGGACAUGCUUcauggcGCGUUAGGa -3' miRNA: 3'- caCGCcuAUCUGUGCGAA------UGCAAUCC- -5' |
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| 10241 | 5' | -48.7 | NC_002686.1 | + | 43879 | 0.78 | 0.648947 |
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Target: 5'- uGUGUGGAUAGACACGCUUAa------ -3' miRNA: 3'- -CACGCCUAUCUGUGCGAAUgcaaucc -5' |
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| 10241 | 5' | -48.7 | NC_002686.1 | + | 43996 | 1.11 | 0.00953 |
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Target: 5'- uGUGCGGAUAGACACGCUUACGUUAGGu -3' miRNA: 3'- -CACGCCUAUCUGUGCGAAUGCAAUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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