Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10242 | 5' | -63 | NC_002686.1 | + | 104832 | 0.66 | 0.560667 |
Target: 5'- -aUCCUCGCGGG-GGCCGuGGugCcCGa -3' miRNA: 3'- ccGGGAGCGUCCaCCGGC-UCugGcGC- -5' |
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10242 | 5' | -63 | NC_002686.1 | + | 123322 | 0.66 | 0.560667 |
Target: 5'- -aUCCUCGCGGG-GGCCGuGGugCcCGa -3' miRNA: 3'- ccGGGAGCGUCCaCCGGC-UCugGcGC- -5' |
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10242 | 5' | -63 | NC_002686.1 | + | 122359 | 0.66 | 0.541451 |
Target: 5'- uGGCCUUUGCGGG-GGCCGucgaauaucuGGGCUccagGCu -3' miRNA: 3'- -CCGGGAGCGUCCaCCGGC----------UCUGG----CGc -5' |
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10242 | 5' | -63 | NC_002686.1 | + | 105796 | 0.66 | 0.541451 |
Target: 5'- uGGCCUUUGCGGG-GGCCGucgaauaucuGGGCUccagGCu -3' miRNA: 3'- -CCGGGAGCGUCCaCCGGC----------UCUGG----CGc -5' |
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10242 | 5' | -63 | NC_002686.1 | + | 123771 | 0.66 | 0.531925 |
Target: 5'- gGGgCCggGUGGaGUGGgCGGGGCCGCu -3' miRNA: 3'- -CCgGGagCGUC-CACCgGCUCUGGCGc -5' |
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10242 | 5' | -63 | NC_002686.1 | + | 104383 | 0.66 | 0.531925 |
Target: 5'- gGGgCCggGUGGaGUGGgCGGGGCCGCu -3' miRNA: 3'- -CCgGGagCGUC-CACCgGCUCUGGCGc -5' |
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10242 | 5' | -63 | NC_002686.1 | + | 121716 | 0.7 | 0.336708 |
Target: 5'- cGGCCC-CGCuguuaAGGcGGCCaaGAGACgCGCGu -3' miRNA: 3'- -CCGGGaGCG-----UCCaCCGG--CUCUG-GCGC- -5' |
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10242 | 5' | -63 | NC_002686.1 | + | 106439 | 0.7 | 0.336708 |
Target: 5'- cGGCCC-CGCuguuaAGGcGGCCaaGAGACgCGCGu -3' miRNA: 3'- -CCGGGaGCG-----UCCaCCGG--CUCUG-GCGC- -5' |
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10242 | 5' | -63 | NC_002686.1 | + | 122918 | 0.76 | 0.152505 |
Target: 5'- aGGCCCggcagCGCGGGU--CCGAGACCGUc -3' miRNA: 3'- -CCGGGa----GCGUCCAccGGCUCUGGCGc -5' |
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10242 | 5' | -63 | NC_002686.1 | + | 105236 | 0.76 | 0.152505 |
Target: 5'- aGGCCCggcagCGCGGGU--CCGAGACCGUc -3' miRNA: 3'- -CCGGGa----GCGUCCAccGGCUCUGGCGc -5' |
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10242 | 5' | -63 | NC_002686.1 | + | 123324 | 1.11 | 0.00045 |
Target: 5'- cGGCCCUCGCAGGUGGCCGAGACCGCGg -3' miRNA: 3'- -CCGGGAGCGUCCACCGGCUCUGGCGC- -5' |
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10242 | 5' | -63 | NC_002686.1 | + | 104831 | 1.11 | 0.00045 |
Target: 5'- cGGCCCUCGCAGGUGGCCGAGACCGCGg -3' miRNA: 3'- -CCGGGAGCGUCCACCGGCUCUGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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