Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10246 | 3' | -53.7 | NC_002686.1 | + | 111445 | 0.66 | 0.944221 |
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Target: 5'- cUCCgcgCCuUCGUCuaccucaacuucggUGUCGUCCUCAa- -3' miRNA: 3'- -AGGa--GG-AGCAGua------------GCAGCAGGAGUag -5' |
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| 10246 | 3' | -53.7 | NC_002686.1 | + | 116710 | 0.66 | 0.944221 |
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Target: 5'- cUCCgcgCCuUCGUCuaccucaacuucggUGUCGUCCUCAa- -3' miRNA: 3'- -AGGa--GG-AGCAGua------------GCAGCAGGAGUag -5' |
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| 10246 | 3' | -53.7 | NC_002686.1 | + | 16672 | 0.67 | 0.923375 |
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Target: 5'- gCUcCCUCaUCAUCGUCGUCCg---- -3' miRNA: 3'- aGGaGGAGcAGUAGCAGCAGGaguag -5' |
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| 10246 | 3' | -53.7 | NC_002686.1 | + | 107240 | 0.69 | 0.847264 |
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Target: 5'- gCCUgCUUGUCc-CGUgUGUCCUCAUCu -3' miRNA: 3'- aGGAgGAGCAGuaGCA-GCAGGAGUAG- -5' |
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| 10246 | 3' | -53.7 | NC_002686.1 | + | 120915 | 0.69 | 0.847264 |
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Target: 5'- gCCUgCUUGUCc-CGUgUGUCCUCAUCu -3' miRNA: 3'- aGGAgGAGCAGuaGCA-GCAGGAGUAG- -5' |
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| 10246 | 3' | -53.7 | NC_002686.1 | + | 120730 | 1.1 | 0.003818 |
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Target: 5'- aUCCUCCUCGUCAUCGUCGUCCUCAUCc -3' miRNA: 3'- -AGGAGGAGCAGUAGCAGCAGGAGUAG- -5' |
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| 10246 | 3' | -53.7 | NC_002686.1 | + | 107425 | 1.1 | 0.003818 |
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Target: 5'- aUCCUCCUCGUCAUCGUCGUCCUCAUCc -3' miRNA: 3'- -AGGAGGAGCAGUAGCAGCAGGAGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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