Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10248 | 5' | -65.5 | NC_002686.1 | + | 123061 | 0.67 | 0.430301 |
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Target: 5'- gGACCCGCGcugccGGgCCUGcGGcCCUCGGg -3' miRNA: 3'- gCUGGGUGC-----CCgGGGCuCCaGGGGCC- -5' |
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| 10248 | 5' | -65.5 | NC_002686.1 | + | 123176 | 0.69 | 0.355023 |
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Target: 5'- gGGCaCCACGGcCCCCGcgaggaugacggugaAGGcgcagCCCCGGg -3' miRNA: 3'- gCUG-GGUGCCcGGGGC---------------UCCa----GGGGCC- -5' |
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| 10248 | 5' | -65.5 | NC_002686.1 | + | 123500 | 0.66 | 0.508709 |
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Target: 5'- gCGGCCCuucacuggagcgACGGGCgCCGcGGUCUCgGc -3' miRNA: 3'- -GCUGGG------------UGCCCGgGGCuCCAGGGgCc -5' |
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| 10248 | 5' | -65.5 | NC_002686.1 | + | 123685 | 0.67 | 0.413851 |
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Target: 5'- -cACCCAcCGGGCgccCCCGGGGggCUCCGc -3' miRNA: 3'- gcUGGGU-GCCCG---GGGCUCCa-GGGGCc -5' |
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| 10248 | 5' | -65.5 | NC_002686.1 | + | 124134 | 0.66 | 0.49968 |
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Target: 5'- cCGGCCCucuccCGGGUCCCGAaaaaaCCGGg -3' miRNA: 3'- -GCUGGGu----GCCCGGGGCUccaggGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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