Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
10249 | 3' | -49.5 | NC_002686.1 | + | 106672 | 0.66 | 0.992061 |
Target: 5'- cGGC----CGUCCCCGCACGGccggGCGg -3' miRNA: 3'- aCUGuuucGCAGGGGCGUGUUaa--UGU- -5' |
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10249 | 3' | -49.5 | NC_002686.1 | + | 121483 | 0.66 | 0.992061 |
Target: 5'- cGGC----CGUCCCCGCACGGccggGCGg -3' miRNA: 3'- aCUGuuucGCAGGGGCGUGUUaa--UGU- -5' |
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10249 | 3' | -49.5 | NC_002686.1 | + | 743 | 0.66 | 0.991946 |
Target: 5'- aGACAAGGCGguauaaaUCUCCGaAUAcgUGCAa -3' miRNA: 3'- aCUGUUUCGC-------AGGGGCgUGUuaAUGU- -5' |
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10249 | 3' | -49.5 | NC_002686.1 | + | 108058 | 0.67 | 0.989507 |
Target: 5'- aGAUcuugcGCGUCCUCGCACAGa---- -3' miRNA: 3'- aCUGuuu--CGCAGGGGCGUGUUaaugu -5' |
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10249 | 3' | -49.5 | NC_002686.1 | + | 120097 | 0.67 | 0.989507 |
Target: 5'- aGAUcuugcGCGUCCUCGCACAGa---- -3' miRNA: 3'- aCUGuuu--CGCAGGGGCGUGUUaaugu -5' |
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10249 | 3' | -49.5 | NC_002686.1 | + | 110400 | 1.1 | 0.008119 |
Target: 5'- cUGACAAAGCGUCCCCGCACAAUUACAg -3' miRNA: 3'- -ACUGUUUCGCAGGGGCGUGUUAAUGU- -5' |
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10249 | 3' | -49.5 | NC_002686.1 | + | 117755 | 1.1 | 0.008119 |
Target: 5'- cUGACAAAGCGUCCCCGCACAAUUACAg -3' miRNA: 3'- -ACUGUUUCGCAGGGGCGUGUUAAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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