Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10249 | 5' | -55 | NC_002686.1 | + | 121744 | 0.65 | 0.897062 |
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Target: 5'- cGCGCGUcGGcuCAGUAAcCGCCUCuGGg -3' miRNA: 3'- uUGCGUAaCCu-GUCGUU-GCGGAG-CCa -5' |
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| 10249 | 5' | -55 | NC_002686.1 | + | 123517 | 0.67 | 0.834701 |
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Target: 5'- aGGCGCggUGGGCgaAGCGgagccccccggggGCGCC-CGGUg -3' miRNA: 3'- -UUGCGuaACCUG--UCGU-------------UGCGGaGCCA- -5' |
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| 10249 | 5' | -55 | NC_002686.1 | + | 104638 | 0.67 | 0.834701 |
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Target: 5'- aGGCGCggUGGGCgaAGCGgagccccccggggGCGCC-CGGUg -3' miRNA: 3'- -UUGCGuaACCUG--UCGU-------------UGCGGaGCCA- -5' |
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| 10249 | 5' | -55 | NC_002686.1 | + | 48662 | 0.79 | 0.241926 |
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Target: 5'- uACGCcUUGGGCAGUAACGCCUUGa- -3' miRNA: 3'- uUGCGuAACCUGUCGUUGCGGAGCca -5' |
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| 10249 | 5' | -55 | NC_002686.1 | + | 110435 | 1.06 | 0.003986 |
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Target: 5'- aAACGCAUUGGACAGCAACGCCUCGGUa -3' miRNA: 3'- -UUGCGUAACCUGUCGUUGCGGAGCCA- -5' |
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| 10249 | 5' | -55 | NC_002686.1 | + | 117720 | 1.06 | 0.003986 |
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Target: 5'- aAACGCAUUGGACAGCAACGCCUCGGUa -3' miRNA: 3'- -UUGCGUAACCUGUCGUUGCGGAGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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