Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10250 | 3' | -55.2 | NC_002686.1 | + | 111428 | 0.66 | 0.904429 |
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Target: 5'- uAUCCAGACCCGccGcaCUcCGCGCCu- -3' miRNA: 3'- -UGGGUUUGGGCuaCa-GAuGCGCGGug -5' |
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| 10250 | 3' | -55.2 | NC_002686.1 | + | 116727 | 0.66 | 0.904429 |
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Target: 5'- uAUCCAGACCCGccGcaCUcCGCGCCu- -3' miRNA: 3'- -UGGGUUUGGGCuaCa-GAuGCGCGGug -5' |
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| 10250 | 3' | -55.2 | NC_002686.1 | + | 107348 | 0.68 | 0.793321 |
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Target: 5'- aACCCAAacGCUCGAgGUUUuccGCGUGCCGg -3' miRNA: 3'- -UGGGUU--UGGGCUaCAGA---UGCGCGGUg -5' |
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| 10250 | 3' | -55.2 | NC_002686.1 | + | 122927 | 0.68 | 0.793321 |
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Target: 5'- aGCgCGggUCCGAgaccGUCcgACGUGCCGCu -3' miRNA: 3'- -UGgGUuuGGGCUa---CAGa-UGCGCGGUG- -5' |
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| 10250 | 3' | -55.2 | NC_002686.1 | + | 105227 | 0.68 | 0.793321 |
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Target: 5'- aGCgCGggUCCGAgaccGUCcgACGUGCCGCu -3' miRNA: 3'- -UGgGUuuGGGCUa---CAGa-UGCGCGGUG- -5' |
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| 10250 | 3' | -55.2 | NC_002686.1 | + | 120807 | 0.68 | 0.793321 |
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Target: 5'- aACCCAAacGCUCGAgGUUUuccGCGUGCCGg -3' miRNA: 3'- -UGGGUU--UGGGCUaCAGA---UGCGCGGUg -5' |
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| 10250 | 3' | -55.2 | NC_002686.1 | + | 91608 | 0.74 | 0.469763 |
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Target: 5'- aACUUAAuCCaCGgcGUCUGCGUGCCACa -3' miRNA: 3'- -UGGGUUuGG-GCuaCAGAUGCGCGGUG- -5' |
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| 10250 | 3' | -55.2 | NC_002686.1 | + | 110075 | 1.1 | 0.002269 |
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Target: 5'- cACCCAAACCCGAUGUCUACGCGCCACg -3' miRNA: 3'- -UGGGUUUGGGCUACAGAUGCGCGGUG- -5' |
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| 10250 | 3' | -55.2 | NC_002686.1 | + | 118080 | 1.1 | 0.002269 |
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Target: 5'- cACCCAAACCCGAUGUCUACGCGCCACg -3' miRNA: 3'- -UGGGUUUGGGCUACAGAUGCGCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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