miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
10251 3' -48 NC_002686.1 + 87077 0.66 0.997937
Target:  5'- aUAGCGUUGU---UGGCGUUGCgAUc- -3'
miRNA:   3'- -GUCGCAAUAauuAUCGCGACGgUGuu -5'
10251 3' -48 NC_002686.1 + 3470 0.66 0.997514
Target:  5'- ----aUUAUUuGUAGCGUUGCCGCGc -3'
miRNA:   3'- gucgcAAUAAuUAUCGCGACGGUGUu -5'
10251 3' -48 NC_002686.1 + 107925 0.67 0.995036
Target:  5'- aGGUGggGU---UAGCGCUGUCACc- -3'
miRNA:   3'- gUCGCaaUAauuAUCGCGACGGUGuu -5'
10251 3' -48 NC_002686.1 + 120229 0.67 0.995036
Target:  5'- aGGUGggGU---UAGCGCUGUCACc- -3'
miRNA:   3'- gUCGCaaUAauuAUCGCGACGGUGuu -5'
10251 3' -48 NC_002686.1 + 111933 0.68 0.9879
Target:  5'- gUAGCGUUGUUAAUGGaa--GCCAUAGu -3'
miRNA:   3'- -GUCGCAAUAAUUAUCgcgaCGGUGUU- -5'
10251 3' -48 NC_002686.1 + 108214 1.07 0.012999
Target:  5'- aCAGCGUUAUUAAUAGCGCUGCCACAAg -3'
miRNA:   3'- -GUCGCAAUAAUUAUCGCGACGGUGUU- -5'
10251 3' -48 NC_002686.1 + 119941 1.07 0.012999
Target:  5'- aCAGCGUUAUUAAUAGCGCUGCCACAAg -3'
miRNA:   3'- -GUCGCAAUAAUUAUCGCGACGGUGUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.