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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10251 | 3' | -48 | NC_002686.1 | + | 120229 | 0.67 | 0.995036 |
|
Target: 5'- aGGUGggGU---UAGCGCUGUCACc- -3' miRNA: 3'- gUCGCaaUAauuAUCGCGACGGUGuu -5' |
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| 10251 | 3' | -48 | NC_002686.1 | + | 119941 | 1.07 | 0.012999 |
|
Target: 5'- aCAGCGUUAUUAAUAGCGCUGCCACAAg -3' miRNA: 3'- -GUCGCAAUAAUUAUCGCGACGGUGUU- -5' |
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| 10251 | 3' | -48 | NC_002686.1 | + | 111933 | 0.68 | 0.9879 |
|
Target: 5'- gUAGCGUUGUUAAUGGaa--GCCAUAGu -3' miRNA: 3'- -GUCGCAAUAAUUAUCgcgaCGGUGUU- -5' |
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| 10251 | 3' | -48 | NC_002686.1 | + | 108214 | 1.07 | 0.012999 |
|
Target: 5'- aCAGCGUUAUUAAUAGCGCUGCCACAAg -3' miRNA: 3'- -GUCGCAAUAAUUAUCGCGACGGUGUU- -5' |
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| 10251 | 3' | -48 | NC_002686.1 | + | 107925 | 0.67 | 0.995036 |
|
Target: 5'- aGGUGggGU---UAGCGCUGUCACc- -3' miRNA: 3'- gUCGCaaUAauuAUCGCGACGGUGuu -5' |
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| 10251 | 3' | -48 | NC_002686.1 | + | 87077 | 0.66 | 0.997937 |
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Target: 5'- aUAGCGUUGU---UGGCGUUGCgAUc- -3' miRNA: 3'- -GUCGCAAUAauuAUCGCGACGgUGuu -5' |
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| 10251 | 3' | -48 | NC_002686.1 | + | 3470 | 0.66 | 0.997514 |
|
Target: 5'- ----aUUAUUuGUAGCGUUGCCGCGc -3' miRNA: 3'- gucgcAAUAAuUAUCGCGACGGUGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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