Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10253 | 3' | -60.9 | NC_002686.1 | + | 123809 | 0.68 | 0.541852 |
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Target: 5'- gCCGCgGGUGGCuGGCGGacgagggacauaCGgagggaUGGGggGGu -3' miRNA: 3'- -GGUG-CCGCCG-CCGCC------------GCa-----ACCCuuCC- -5' |
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| 10253 | 3' | -60.9 | NC_002686.1 | + | 104345 | 0.68 | 0.541852 |
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Target: 5'- gCCGCgGGUGGCuGGCGGacgagggacauaCGgagggaUGGGggGGu -3' miRNA: 3'- -GGUG-CCGCCG-CCGCC------------GCa-----ACCCuuCC- -5' |
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| 10253 | 3' | -60.9 | NC_002686.1 | + | 123905 | 0.72 | 0.336425 |
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Target: 5'- gCCugGGCGGCaGCGGUcaacGgcGGGguGGa -3' miRNA: 3'- -GGugCCGCCGcCGCCG----CaaCCCuuCC- -5' |
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| 10253 | 3' | -60.9 | NC_002686.1 | + | 104249 | 0.72 | 0.336425 |
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Target: 5'- gCCugGGCGGCaGCGGUcaacGgcGGGguGGa -3' miRNA: 3'- -GGugCCGCCGcCGCCG----CaaCCCuuCC- -5' |
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| 10253 | 3' | -60.9 | NC_002686.1 | + | 106575 | 0.98 | 0.00607 |
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Target: 5'- gCCACGGCGGCGGCGGCGUguGGAAGGg -3' miRNA: 3'- -GGUGCCGCCGCCGCCGCAacCCUUCC- -5' |
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| 10253 | 3' | -60.9 | NC_002686.1 | + | 121580 | 0.98 | 0.00607 |
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Target: 5'- gCCACGGCGGCGGCGGCGUguGGAAGGg -3' miRNA: 3'- -GGUGCCGCCGCCGCCGCAacCCUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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