miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
10253 3' -60.9 NC_002686.1 + 123809 0.68 0.541852
Target:  5'- gCCGCgGGUGGCuGGCGGacgagggacauaCGgagggaUGGGggGGu -3'
miRNA:   3'- -GGUG-CCGCCG-CCGCC------------GCa-----ACCCuuCC- -5'
10253 3' -60.9 NC_002686.1 + 104345 0.68 0.541852
Target:  5'- gCCGCgGGUGGCuGGCGGacgagggacauaCGgagggaUGGGggGGu -3'
miRNA:   3'- -GGUG-CCGCCG-CCGCC------------GCa-----ACCCuuCC- -5'
10253 3' -60.9 NC_002686.1 + 123905 0.72 0.336425
Target:  5'- gCCugGGCGGCaGCGGUcaacGgcGGGguGGa -3'
miRNA:   3'- -GGugCCGCCGcCGCCG----CaaCCCuuCC- -5'
10253 3' -60.9 NC_002686.1 + 104249 0.72 0.336425
Target:  5'- gCCugGGCGGCaGCGGUcaacGgcGGGguGGa -3'
miRNA:   3'- -GGugCCGCCGcCGCCG----CaaCCCuuCC- -5'
10253 3' -60.9 NC_002686.1 + 106575 0.98 0.00607
Target:  5'- gCCACGGCGGCGGCGGCGUguGGAAGGg -3'
miRNA:   3'- -GGUGCCGCCGCCGCCGCAacCCUUCC- -5'
10253 3' -60.9 NC_002686.1 + 121580 0.98 0.00607
Target:  5'- gCCACGGCGGCGGCGGCGUguGGAAGGg -3'
miRNA:   3'- -GGUGCCGCCGCCGCCGCAacCCUUCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.