Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10255 | 3' | -61.2 | NC_002686.1 | + | 123348 | 0.66 | 0.612668 |
Target: 5'- nCGGCgCCCGUcgcuccagugAAGGGCCGcGGgugcccggCUCGUc -3' miRNA: 3'- -GCCGgGGGCG----------UUUCCGGU-CCa-------GAGCG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 106671 | 0.66 | 0.662623 |
Target: 5'- aCGGCcguCCCCGCAc-GGCCGGG-CgggaGUa -3' miRNA: 3'- -GCCG---GGGGCGUuuCCGGUCCaGag--CG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 121484 | 0.66 | 0.662623 |
Target: 5'- aCGGCcguCCCCGCAc-GGCCGGG-CgggaGUa -3' miRNA: 3'- -GCCG---GGGGCGUuuCCGGUCCaGag--CG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 104807 | 0.66 | 0.612668 |
Target: 5'- nCGGCgCCCGUcgcuccagugAAGGGCCGcGGgugcccggCUCGUc -3' miRNA: 3'- -GCCGgGGGCG----------UUUCCGGU-CCa-------GAGCG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 105796 | 0.71 | 0.350267 |
Target: 5'- uGGCCUuuGCGGGGGCCGucgaauaucuGGgcuccaggCUCGCc -3' miRNA: 3'- gCCGGGggCGUUUCCGGU----------CCa-------GAGCG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 122359 | 0.71 | 0.350267 |
Target: 5'- uGGCCUuuGCGGGGGCCGucgaauaucuGGgcuccaggCUCGCc -3' miRNA: 3'- gCCGGGggCGUUUCCGGU----------CCa-------GAGCG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 123323 | 0.71 | 0.350267 |
Target: 5'- cCGGCCCUCGCAGGuGGCCGaGaC-CGCg -3' miRNA: 3'- -GCCGGGGGCGUUU-CCGGUcCaGaGCG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 123183 | 0.71 | 0.372845 |
Target: 5'- aCGGCCCCCGCGAggaugacggugaAGGCgcagcccCGGGUUgCGg -3' miRNA: 3'- -GCCGGGGGCGUU------------UCCG-------GUCCAGaGCg -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 104972 | 0.71 | 0.372845 |
Target: 5'- aCGGCCCCCGCGAggaugacggugaAGGCgcagcccCGGGUUgCGg -3' miRNA: 3'- -GCCGGGGGCGUU------------UCCG-------GUCCAGaGCg -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 104832 | 0.71 | 0.350267 |
Target: 5'- cCGGCCCUCGCAGGuGGCCGaGaC-CGCg -3' miRNA: 3'- -GCCGGGGGCGUUU-CCGGUcCaGaGCG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 32355 | 0.72 | 0.320752 |
Target: 5'- uGGCCCUUuUAGAGGCgGGGUUUaCGCg -3' miRNA: 3'- gCCGGGGGcGUUUCCGgUCCAGA-GCG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 123041 | 0.75 | 0.191589 |
Target: 5'- gCGGCCCUCGgGGAGGUagaCGGGUCgcaggggCGCg -3' miRNA: 3'- -GCCGGGGGCgUUUCCG---GUCCAGa------GCG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 105114 | 0.75 | 0.191589 |
Target: 5'- gCGGCCCUCGgGGAGGUagaCGGGUCgcaggggCGCg -3' miRNA: 3'- -GCCGGGGGCgUUUCCG---GUCCAGa------GCG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 105620 | 0.77 | 0.142289 |
Target: 5'- uGGCCUCgCGCAacuGAGGCC-GGUCUgGCa -3' miRNA: 3'- gCCGGGG-GCGU---UUCCGGuCCAGAgCG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 122535 | 0.77 | 0.142289 |
Target: 5'- uGGCCUCgCGCAacuGAGGCC-GGUCUgGCa -3' miRNA: 3'- gCCGGGG-GCGU---UUCCGGuCCAGAgCG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 123466 | 0.88 | 0.024467 |
Target: 5'- uCGGCCaCCUGCGAGGGCCGGGUCgucccacUCGCa -3' miRNA: 3'- -GCCGG-GGGCGUUUCCGGUCCAG-------AGCG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 104689 | 0.88 | 0.024467 |
Target: 5'- uCGGCCaCCUGCGAGGGCCGGGUCgucccacUCGCa -3' miRNA: 3'- -GCCGG-GGGCGUUUCCGGUCCAG-------AGCG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 105655 | 1.12 | 0.000496 |
Target: 5'- aCGGCCCCCGCAAAGGCCAGGUCUCGCg -3' miRNA: 3'- -GCCGGGGGCGUUUCCGGUCCAGAGCG- -5' |
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10255 | 3' | -61.2 | NC_002686.1 | + | 122500 | 1.12 | 0.000496 |
Target: 5'- aCGGCCCCCGCAAAGGCCAGGUCUCGCg -3' miRNA: 3'- -GCCGGGGGCGUUUCCGGUCCAGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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