Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10256 | 3' | -57.8 | NC_002686.1 | + | 122496 | 0.66 | 0.744162 |
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Target: 5'- aAAGCGCCuGCgcg-GCCAGACgcgcaGGCCg -3' miRNA: 3'- -UUUGUGG-CGagaaCGGUCUGg----CCGGa -5' |
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| 10256 | 3' | -57.8 | NC_002686.1 | + | 105659 | 0.66 | 0.744162 |
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Target: 5'- aAAGCGCCuGCgcg-GCCAGACgcgcaGGCCg -3' miRNA: 3'- -UUUGUGG-CGagaaCGGUCUGg----CCGGa -5' |
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| 10256 | 3' | -57.8 | NC_002686.1 | + | 105093 | 0.66 | 0.734227 |
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Target: 5'- cGGAC-CCGCgc-UGCCGGGCCugcGGCCc -3' miRNA: 3'- -UUUGuGGCGagaACGGUCUGG---CCGGa -5' |
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| 10256 | 3' | -57.8 | NC_002686.1 | + | 123062 | 0.66 | 0.734227 |
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Target: 5'- cGGAC-CCGCgc-UGCCGGGCCugcGGCCc -3' miRNA: 3'- -UUUGuGGCGagaACGGUCUGG---CCGGa -5' |
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| 10256 | 3' | -57.8 | NC_002686.1 | + | 103266 | 0.67 | 0.714091 |
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Target: 5'- aAGACGCUGCUgUUGUCGGG--GGCCa -3' miRNA: 3'- -UUUGUGGCGAgAACGGUCUggCCGGa -5' |
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| 10256 | 3' | -57.8 | NC_002686.1 | + | 86846 | 0.69 | 0.558876 |
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Target: 5'- cAAACACgGCUCgaGCCGGGCCaacaaGGCg- -3' miRNA: 3'- -UUUGUGgCGAGaaCGGUCUGG-----CCGga -5' |
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| 10256 | 3' | -57.8 | NC_002686.1 | + | 37379 | 0.69 | 0.545619 |
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Target: 5'- gAAAUGCCGCUUUUGUUAGACUacgaaauauuuuaaGGCCc -3' miRNA: 3'- -UUUGUGGCGAGAACGGUCUGG--------------CCGGa -5' |
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| 10256 | 3' | -57.8 | NC_002686.1 | + | 105455 | 1.05 | 0.002519 |
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Target: 5'- cAAACACCGCUCUUGCCAGACCGGCCUc -3' miRNA: 3'- -UUUGUGGCGAGAACGGUCUGGCCGGA- -5' |
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| 10256 | 3' | -57.8 | NC_002686.1 | + | 122700 | 1.05 | 0.002519 |
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Target: 5'- cAAACACCGCUCUUGCCAGACCGGCCUc -3' miRNA: 3'- -UUUGUGGCGAGAACGGUCUGGCCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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