Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10257 | 3' | -57.4 | NC_002686.1 | + | 105339 | 0.66 | 0.845663 |
Target: 5'- gGGGGCGGCGGAcUUCGUugaCGAugcGGCa -3' miRNA: 3'- -CCCUGUCGCCUuGGGCGcaaGCU---CCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 122815 | 0.66 | 0.845663 |
Target: 5'- gGGGGCGGCGGAcUUCGUugaCGAugcGGCa -3' miRNA: 3'- -CCCUGUCGCCUuGGGCGcaaGCU---CCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 104785 | 0.66 | 0.837633 |
Target: 5'- aGGGCcGCGGGuGCCCGgcucguCGUaCGGGGUg -3' miRNA: 3'- cCCUGuCGCCU-UGGGC------GCAaGCUCCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 123370 | 0.66 | 0.837633 |
Target: 5'- aGGGCcGCGGGuGCCCGgcucguCGUaCGGGGUg -3' miRNA: 3'- cCCUGuCGCCU-UGGGC------GCAaGCUCCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 286 | 0.66 | 0.829419 |
Target: 5'- cGGGCGGUGGGuAgCCGCGgacauGGCg -3' miRNA: 3'- cCCUGUCGCCU-UgGGCGCaagcuCCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 117244 | 0.67 | 0.803756 |
Target: 5'- aGGG-UAGCaGGAACCCGgGUUgaCGGguucGGCc -3' miRNA: 3'- -CCCuGUCG-CCUUGGGCgCAA--GCU----CCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 110911 | 0.67 | 0.803756 |
Target: 5'- aGGG-UAGCaGGAACCCGgGUUgaCGGguucGGCc -3' miRNA: 3'- -CCCuGUCG-CCUUGGGCgCAA--GCU----CCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 104407 | 0.68 | 0.742805 |
Target: 5'- gGGGugGGCGGGuccgccaaucgggggGCCggguggaGUGggCGGGGCc -3' miRNA: 3'- -CCCugUCGCCU---------------UGGg------CGCaaGCUCCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 123072 | 0.68 | 0.738953 |
Target: 5'- cGGACGGUcucGGACCCGCGcugCcGGGCc -3' miRNA: 3'- cCCUGUCGc--CUUGGGCGCaa-GcUCCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 105083 | 0.68 | 0.738953 |
Target: 5'- cGGACGGUcucGGACCCGCGcugCcGGGCc -3' miRNA: 3'- cCCUGUCGc--CUUGGGCGCaa-GcUCCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 123150 | 0.68 | 0.709636 |
Target: 5'- uGGGGgA-UGGAACCCGUcugguGUUCG-GGCa -3' miRNA: 3'- -CCCUgUcGCCUUGGGCG-----CAAGCuCCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 105005 | 0.68 | 0.709636 |
Target: 5'- uGGGGgA-UGGAACCCGUcugguGUUCG-GGCa -3' miRNA: 3'- -CCCUgUcGCCUUGGGCG-----CAAGCuCCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 1208 | 0.7 | 0.63938 |
Target: 5'- cGGGGCAuGUGaGGCCgGCGUguUgGGGGCg -3' miRNA: 3'- -CCCUGU-CGCcUUGGgCGCA--AgCUCCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 123805 | 0.71 | 0.539087 |
Target: 5'- gGGGGCcGCGGGugGCUgGCGgaCGAGGg -3' miRNA: 3'- -CCCUGuCGCCU--UGGgCGCaaGCUCCg -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 104349 | 0.71 | 0.539087 |
Target: 5'- gGGGGCcGCGGGugGCUgGCGgaCGAGGg -3' miRNA: 3'- -CCCUGuCGCCU--UGGgCGCaaGCUCCg -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 120300 | 0.72 | 0.50991 |
Target: 5'- gGGGACcuCGGGGCCCGUGggUCGcuuGGUc -3' miRNA: 3'- -CCCUGucGCCUUGGGCGCa-AGCu--CCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 107854 | 0.72 | 0.50991 |
Target: 5'- gGGGACcuCGGGGCCCGUGggUCGcuuGGUc -3' miRNA: 3'- -CCCUGucGCCUUGGGCGCa-AGCu--CCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 105295 | 1.12 | 0.001236 |
Target: 5'- gGGGACAGCGGAACCCGCGUUCGAGGCc -3' miRNA: 3'- -CCCUGUCGCCUUGGGCGCAAGCUCCG- -5' |
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10257 | 3' | -57.4 | NC_002686.1 | + | 122860 | 1.12 | 0.001236 |
Target: 5'- gGGGACAGCGGAACCCGCGUUCGAGGCc -3' miRNA: 3'- -CCCUGUCGCCUUGGGCGCAAGCUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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