Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10258 | 3' | -62.2 | NC_002686.1 | + | 123808 | 0.68 | 0.494476 |
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Target: 5'- gGCCGCGGg--UGGCUGgCgGaCGAGGGa -3' miRNA: 3'- -CGGCGCCagaGCCGGUgGaC-GCUCCC- -5' |
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| 10258 | 3' | -62.2 | NC_002686.1 | + | 104346 | 0.68 | 0.494476 |
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Target: 5'- gGCCGCGGg--UGGCUGgCgGaCGAGGGa -3' miRNA: 3'- -CGGCGCCagaGCCGGUgGaC-GCUCCC- -5' |
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| 10258 | 3' | -62.2 | NC_002686.1 | + | 105080 | 0.69 | 0.415104 |
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Target: 5'- cGUCGgaCGGUCUCGGaCCcgcgcugccggGCCUGCGgcccucGGGGa -3' miRNA: 3'- -CGGC--GCCAGAGCC-GG-----------UGGACGC------UCCC- -5' |
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| 10258 | 3' | -62.2 | NC_002686.1 | + | 123075 | 0.69 | 0.415104 |
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Target: 5'- cGUCGgaCGGUCUCGGaCCcgcgcugccggGCCUGCGgcccucGGGGa -3' miRNA: 3'- -CGGC--GCCAGAGCC-GG-----------UGGACGC------UCCC- -5' |
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| 10258 | 3' | -62.2 | NC_002686.1 | + | 106282 | 0.71 | 0.336708 |
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Target: 5'- -aCGCGcGUCUCuuGGCCGCCUuaacaGCGGGGc -3' miRNA: 3'- cgGCGC-CAGAG--CCGGUGGA-----CGCUCCc -5' |
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| 10258 | 3' | -62.2 | NC_002686.1 | + | 121873 | 0.71 | 0.336708 |
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Target: 5'- -aCGCGcGUCUCuuGGCCGCCUuaacaGCGGGGc -3' miRNA: 3'- cgGCGC-CAGAG--CCGGUGGA-----CGCUCCc -5' |
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| 10258 | 3' | -62.2 | NC_002686.1 | + | 104679 | 1.11 | 0.000573 |
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Target: 5'- cGCCGCGGUCUCGGCCACCUGCGAGGGc -3' miRNA: 3'- -CGGCGCCAGAGCCGGUGGACGCUCCC- -5' |
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| 10258 | 3' | -62.2 | NC_002686.1 | + | 123476 | 1.11 | 0.000573 |
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Target: 5'- cGCCGCGGUCUCGGCCACCUGCGAGGGc -3' miRNA: 3'- -CGGCGCCAGAGCCGGUGGACGCUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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