Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
10262 | 3' | -43.7 | NC_002686.1 | + | 107898 | 0.67 | 0.999997 |
Target: 5'- cUCCgauggUCUUgcUGUugGUGAUGUAGGuGGGg -3' miRNA: 3'- -AGG-----AGAAauAUA--UACUGUAUCCuCCC- -5' |
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10262 | 3' | -43.7 | NC_002686.1 | + | 120256 | 0.67 | 0.999997 |
Target: 5'- cUCCgauggUCUUgcUGUugGUGAUGUAGGuGGGg -3' miRNA: 3'- -AGG-----AGAAauAUA--UACUGUAUCCuCCC- -5' |
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10262 | 3' | -43.7 | NC_002686.1 | + | 123481 | 0.69 | 0.999845 |
Target: 5'- gUCCUCguccuccUcgGACG-AGGAGGGg -3' miRNA: 3'- -AGGAGaaauau-AuaCUGUaUCCUCCC- -5' |
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10262 | 3' | -43.7 | NC_002686.1 | + | 104674 | 0.69 | 0.999845 |
Target: 5'- gUCCUCguccuccUcgGACG-AGGAGGGg -3' miRNA: 3'- -AGGAGaaauau-AuaCUGUaUCCUCCC- -5' |
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10262 | 3' | -43.7 | NC_002686.1 | + | 44943 | 0.86 | 0.608526 |
Target: 5'- aCgUUUUUAaugcUAUAUGACAUAGGAGGGg -3' miRNA: 3'- aGgAGAAAU----AUAUACUGUAUCCUCCC- -5' |
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10262 | 3' | -43.7 | NC_002686.1 | + | 44753 | 1.15 | 0.018803 |
Target: 5'- uUCCUCUUUAUAUAUGACAUAGGAGGGg -3' miRNA: 3'- -AGGAGAAAUAUAUACUGUAUCCUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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