Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10264 | 5' | -45.8 | NC_002686.1 | + | 60400 | 0.66 | 0.999799 |
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Target: 5'- -aGCUcgGCauuGCGAgGGUCCCAGAGg -3' miRNA: 3'- aaUGAaaCGg--UGUUgCUAGGGUUUUg -5' |
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| 10264 | 5' | -45.8 | NC_002686.1 | + | 16270 | 0.66 | 0.999739 |
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Target: 5'- uUUGCcgUGCUACAGCuauguaucugGGUCCCccAGAGCa -3' miRNA: 3'- -AAUGaaACGGUGUUG----------CUAGGG--UUUUG- -5' |
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| 10264 | 5' | -45.8 | NC_002686.1 | + | 39626 | 0.67 | 0.999318 |
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Target: 5'- ----aUUGCCAUGcCGuAUCCCAAGAUa -3' miRNA: 3'- aaugaAACGGUGUuGC-UAGGGUUUUG- -5' |
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| 10264 | 5' | -45.8 | NC_002686.1 | + | 121571 | 0.67 | 0.999149 |
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Target: 5'- -cACgg-GCCGCGguaGCGGcCCCGGAACc -3' miRNA: 3'- aaUGaaaCGGUGU---UGCUaGGGUUUUG- -5' |
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| 10264 | 5' | -45.8 | NC_002686.1 | + | 106584 | 0.67 | 0.999149 |
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Target: 5'- -cACgg-GCCGCGguaGCGGcCCCGGAACc -3' miRNA: 3'- aaUGaaaCGGUGU---UGCUaGGGUUUUG- -5' |
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| 10264 | 5' | -45.8 | NC_002686.1 | + | 74881 | 0.68 | 0.998062 |
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Target: 5'- gUAUUUgucCCACGaaauACGAUUCCGAAGCg -3' miRNA: 3'- aAUGAAac-GGUGU----UGCUAGGGUUUUG- -5' |
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| 10264 | 5' | -45.8 | NC_002686.1 | + | 49753 | 0.7 | 0.991033 |
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Target: 5'- -gACUUUGCUGCGAUGAuuaaacccaccuuUCCCAAc-- -3' miRNA: 3'- aaUGAAACGGUGUUGCU-------------AGGGUUuug -5' |
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| 10264 | 5' | -45.8 | NC_002686.1 | + | 778 | 0.7 | 0.989649 |
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Target: 5'- aUAUaaugGCUGCAACGAccacaagUCCCAAAACa -3' miRNA: 3'- aAUGaaa-CGGUGUUGCU-------AGGGUUUUG- -5' |
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| 10264 | 5' | -45.8 | NC_002686.1 | + | 84343 | 1.1 | 0.017923 |
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Target: 5'- uUUACUUUGCCACAACGAUCCCAAAACa -3' miRNA: 3'- -AAUGAAACGGUGUUGCUAGGGUUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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