miRNA: 3'- -CCCUUAAACUUUuuUCAGaggUUCCa -5'
miRNA: 3'- -CCCUUAAACUU-UUUUcAGAGGuuCCA- -5'
miRNA: 3'- -CCCUUAAACUUUUUUCAGAGGUUCCA- -5'
miRNA: 3'- -CCCUUAAACUUUUUUCAGAGGUUCCA- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10277 | 5' | -41.9 | NC_002686.1 | + | 74270 | 0.67 | 1 |
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Target: 5'- aGGGAGUUUGGAAccAGUUgauGAGGa -3' miRNA: 3'- -CCCUUAAACUUUuuUCAGaggUUCCa -5' |
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| 10277 | 5' | -41.9 | NC_002686.1 | + | 85716 | 0.68 | 0.999999 |
|
Target: 5'- cGGGAAUUUGAAcAAAAcUUUCCccGGUg -3' miRNA: 3'- -CCCUUAAACUU-UUUUcAGAGGuuCCA- -5' |
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| 10277 | 5' | -41.9 | NC_002686.1 | + | 120403 | 1.13 | 0.037566 |
|
Target: 5'- gGGGAAUUUGAAAAAAGUCUCCAAGGUg -3' miRNA: 3'- -CCCUUAAACUUUUUUCAGAGGUUCCA- -5' |
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| 10277 | 5' | -41.9 | NC_002686.1 | + | 107752 | 1.13 | 0.037566 |
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Target: 5'- gGGGAAUUUGAAAAAAGUCUCCAAGGUg -3' miRNA: 3'- -CCCUUAAACUUUUUUCAGAGGUUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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