Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1028 | 3' | -60.4 | NC_000924.1 | + | 23369 | 0.66 | 0.487418 |
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Target: 5'- cUCAGCGCCUUCUGUUgCGCCA-GAa-- -3' miRNA: 3'- -AGUCGCGGGGGACGA-GUGGUgCUgcc -5' |
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| 1028 | 3' | -60.4 | NC_000924.1 | + | 39188 | 0.66 | 0.46806 |
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Target: 5'- gCAGCGCUgCCUGCcggaccuggcUCuCCACGA-GGu -3' miRNA: 3'- aGUCGCGGgGGACG----------AGuGGUGCUgCC- -5' |
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| 1028 | 3' | -60.4 | NC_000924.1 | + | 34742 | 0.67 | 0.434251 |
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Target: 5'- uUUGGCGCUUCCUGCUgucagugaggauauuUACCAuCGAgCGGu -3' miRNA: 3'- -AGUCGCGGGGGACGA---------------GUGGU-GCU-GCC- -5' |
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| 1028 | 3' | -60.4 | NC_000924.1 | + | 26595 | 0.69 | 0.34515 |
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Target: 5'- cCAGCGCgCUCUcCUgGUCACGACGGg -3' miRNA: 3'- aGUCGCGgGGGAcGAgUGGUGCUGCC- -5' |
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| 1028 | 3' | -60.4 | NC_000924.1 | + | 6229 | 0.69 | 0.32959 |
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Target: 5'- --uGCGCaCCgCCUGCUCuCC-CGAUGGu -3' miRNA: 3'- aguCGCG-GG-GGACGAGuGGuGCUGCC- -5' |
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| 1028 | 3' | -60.4 | NC_000924.1 | + | 56154 | 0.7 | 0.286055 |
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Target: 5'- gCGGCGUCUCUUGC-CAaaaaCAUGACGGu -3' miRNA: 3'- aGUCGCGGGGGACGaGUg---GUGCUGCC- -5' |
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| 1028 | 3' | -60.4 | NC_000924.1 | + | 38894 | 0.7 | 0.279256 |
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Target: 5'- --cGCuCCCCCUGUucgcccuucUCACCACG-CGGa -3' miRNA: 3'- aguCGcGGGGGACG---------AGUGGUGCuGCC- -5' |
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| 1028 | 3' | -60.4 | NC_000924.1 | + | 49429 | 0.73 | 0.173394 |
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Target: 5'- aUCAGC-CCCCCUGCU-GCCAcCGGCa- -3' miRNA: 3'- -AGUCGcGGGGGACGAgUGGU-GCUGcc -5' |
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| 1028 | 3' | -60.4 | NC_000924.1 | + | 56279 | 1.1 | 0.000358 |
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Target: 5'- aUCAGCGCCCCCUGCUCACCACGACGGu -3' miRNA: 3'- -AGUCGCGGGGGACGAGUGGUGCUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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