Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10284 | 5' | -54.4 | NC_002687.1 | + | 238105 | 0.66 | 0.990341 |
Target: 5'- gCUGGCauuccUCUgauCUCGGCGUCGGUc- -3' miRNA: 3'- -GACCGca---AGAau-GAGCUGCGGCCAca -5' |
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10284 | 5' | -54.4 | NC_002687.1 | + | 56871 | 0.66 | 0.990341 |
Target: 5'- cCUGcGCGUguaUGCcgCGGCGCCGGUu- -3' miRNA: 3'- -GAC-CGCAagaAUGa-GCUGCGGCCAca -5' |
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10284 | 5' | -54.4 | NC_002687.1 | + | 8162 | 0.67 | 0.989088 |
Target: 5'- -cGGCGUcgUUguagugACUCGGgGCCuGGUGUa -3' miRNA: 3'- gaCCGCA--AGaa----UGAGCUgCGG-CCACA- -5' |
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10284 | 5' | -54.4 | NC_002687.1 | + | 263605 | 0.67 | 0.987712 |
Target: 5'- --uGCGUcgaucgcaUCUUcucgaGCUUGGCGCUGGUGUa -3' miRNA: 3'- gacCGCA--------AGAA-----UGAGCUGCGGCCACA- -5' |
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10284 | 5' | -54.4 | NC_002687.1 | + | 125068 | 0.67 | 0.986206 |
Target: 5'- uCUGGCGUUU---CUCGACGCCu---- -3' miRNA: 3'- -GACCGCAAGaauGAGCUGCGGccaca -5' |
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10284 | 5' | -54.4 | NC_002687.1 | + | 210404 | 0.68 | 0.980832 |
Target: 5'- gUGGUcUUCUUGCUauuuuucgaCGACGCCGGc-- -3' miRNA: 3'- gACCGcAAGAAUGA---------GCUGCGGCCaca -5' |
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10284 | 5' | -54.4 | NC_002687.1 | + | 117958 | 0.68 | 0.978732 |
Target: 5'- -cGGUGUUCaucacACcCGGCGCgGGUGUa -3' miRNA: 3'- gaCCGCAAGaa---UGaGCUGCGgCCACA- -5' |
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10284 | 5' | -54.4 | NC_002687.1 | + | 239537 | 0.71 | 0.919084 |
Target: 5'- -gGGUGUUgaUGCg-GGCGCCGGUGa -3' miRNA: 3'- gaCCGCAAgaAUGagCUGCGGCCACa -5' |
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10284 | 5' | -54.4 | NC_002687.1 | + | 302274 | 0.72 | 0.889286 |
Target: 5'- gCUGGCGU----GCUCGuauaGCCGGUGg -3' miRNA: 3'- -GACCGCAagaaUGAGCug--CGGCCACa -5' |
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10284 | 5' | -54.4 | NC_002687.1 | + | 28614 | 0.72 | 0.882692 |
Target: 5'- aCUGGgGaacUCggcaggUGCUCGGCgGCCGGUGUc -3' miRNA: 3'- -GACCgCa--AGa-----AUGAGCUG-CGGCCACA- -5' |
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10284 | 5' | -54.4 | NC_002687.1 | + | 295148 | 0.72 | 0.868897 |
Target: 5'- aUGGCGUcCUgcaGCacaCGGCGCCGGUGc -3' miRNA: 3'- gACCGCAaGAa--UGa--GCUGCGGCCACa -5' |
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10284 | 5' | -54.4 | NC_002687.1 | + | 3169 | 1.07 | 0.01349 |
Target: 5'- uCUGGCGUUCUUACUCGACGCCGGUGUc -3' miRNA: 3'- -GACCGCAAGAAUGAGCUGCGGCCACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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