Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10285 | 3' | -56.2 | NC_002687.1 | + | 110172 | 0.66 | 0.980435 |
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Target: 5'- gAUCGGUGGaUGAGG--UGGCCGUUg- -3' miRNA: 3'- -UAGCCACCaGCUUUggACCGGCAGug -5' |
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| 10285 | 3' | -56.2 | NC_002687.1 | + | 296177 | 0.66 | 0.978311 |
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Target: 5'- uUCGGUGG-CGGAGCUgaGGgUGUUGCa -3' miRNA: 3'- uAGCCACCaGCUUUGGa-CCgGCAGUG- -5' |
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| 10285 | 3' | -56.2 | NC_002687.1 | + | 305295 | 0.67 | 0.947021 |
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Target: 5'- uUCGGUGGUgcauggUGAugAACCUGGUCGgccgaGCg -3' miRNA: 3'- uAGCCACCA------GCU--UUGGACCGGCag---UG- -5' |
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| 10285 | 3' | -56.2 | NC_002687.1 | + | 290623 | 0.68 | 0.923673 |
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Target: 5'- cUUGGcGGUCGGAcCCUGGgCGUUAg -3' miRNA: 3'- uAGCCaCCAGCUUuGGACCgGCAGUg -5' |
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| 10285 | 3' | -56.2 | NC_002687.1 | + | 172437 | 0.7 | 0.881931 |
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Target: 5'- -gUGGUGcGUCGGcauccGACUUGGCCGaCGCu -3' miRNA: 3'- uaGCCAC-CAGCU-----UUGGACCGGCaGUG- -5' |
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| 10285 | 3' | -56.2 | NC_002687.1 | + | 215697 | 0.7 | 0.868141 |
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Target: 5'- cGUCGGUGGcggucgUCGuAAugUcacuggucgUGGCCGUCACu -3' miRNA: 3'- -UAGCCACC------AGC-UUugG---------ACCGGCAGUG- -5' |
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| 10285 | 3' | -56.2 | NC_002687.1 | + | 115538 | 0.7 | 0.868141 |
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Target: 5'- -cCGGUGGcaUCGGAGCuCUGGCgugCGUUGCg -3' miRNA: 3'- uaGCCACC--AGCUUUG-GACCG---GCAGUG- -5' |
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| 10285 | 3' | -56.2 | NC_002687.1 | + | 3612 | 1.06 | 0.008741 |
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Target: 5'- cAUCGGUGGUCGAAACCUGGCCGUCACc -3' miRNA: 3'- -UAGCCACCAGCUUUGGACCGGCAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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