Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10286 | 3' | -53.5 | NC_002687.1 | + | 45765 | 0.73 | 0.875717 |
Target: 5'- aCGCuUCGUCGA-GAGCAUuuUGCGCACc -3' miRNA: 3'- -GUGcAGUAGCUgCUCGUGu-ACGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 40406 | 0.73 | 0.860599 |
Target: 5'- cCAUGUCcuGUCGGCGGGgGCGuugaccaaaagcuUGCGCACc -3' miRNA: 3'- -GUGCAG--UAGCUGCUCgUGU-------------ACGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 80118 | 0.74 | 0.822096 |
Target: 5'- gCGCGUCAggaUCGACGAcCAUcaGCGCACa -3' miRNA: 3'- -GUGCAGU---AGCUGCUcGUGuaCGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 161408 | 0.74 | 0.813734 |
Target: 5'- -cCGUCGUCGGaacuguCGaAGCACGUGCGCGa -3' miRNA: 3'- guGCAGUAGCU------GC-UCGUGUACGCGUg -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 4335 | 1.11 | 0.008477 |
Target: 5'- aCACGUCAUCGACGAGCACAUGCGCACg -3' miRNA: 3'- -GUGCAGUAGCUGCUCGUGUACGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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