Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10286 | 5' | -51.3 | NC_002687.1 | + | 305638 | 0.66 | 0.99848 |
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Target: 5'- ---gGCgACcGaGCCCUCUAUGAUGUg -3' miRNA: 3'- ucuaCG-UGuCaCGGGAGAUACUACAg -5' |
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| 10286 | 5' | -51.3 | NC_002687.1 | + | 207428 | 0.67 | 0.997446 |
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Target: 5'- cAGAucaUGCACaagGGUGCCCUCUAUa----- -3' miRNA: 3'- -UCU---ACGUG---UCACGGGAGAUAcuacag -5' |
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| 10286 | 5' | -51.3 | NC_002687.1 | + | 201443 | 0.67 | 0.996474 |
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Target: 5'- cAGAucaUGCACaaaGGUGCCCUCUccGAccUCg -3' miRNA: 3'- -UCU---ACGUG---UCACGGGAGAuaCUacAG- -5' |
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| 10286 | 5' | -51.3 | NC_002687.1 | + | 319651 | 0.7 | 0.982528 |
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Target: 5'- aGGGUGCGCuucAGUGgUUUCUGUGA-GUCg -3' miRNA: 3'- -UCUACGUG---UCACgGGAGAUACUaCAG- -5' |
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| 10286 | 5' | -51.3 | NC_002687.1 | + | 246264 | 0.71 | 0.967484 |
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Target: 5'- aGGGUGCGCuucAGUGgUUUCUGUGAgGUCg -3' miRNA: 3'- -UCUACGUG---UCACgGGAGAUACUaCAG- -5' |
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| 10286 | 5' | -51.3 | NC_002687.1 | + | 109140 | 0.71 | 0.964285 |
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Target: 5'- uGGUGCcauCGGUGCUCUCgGUGGUGcUCu -3' miRNA: 3'- uCUACGu--GUCACGGGAGaUACUAC-AG- -5' |
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| 10286 | 5' | -51.3 | NC_002687.1 | + | 315117 | 0.73 | 0.908175 |
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Target: 5'- ---cGCGCAGcugccucaauUGCCCUCcugAUGAUGUCu -3' miRNA: 3'- ucuaCGUGUC----------ACGGGAGa--UACUACAG- -5' |
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| 10286 | 5' | -51.3 | NC_002687.1 | + | 4300 | 1.11 | 0.011482 |
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Target: 5'- uAGAUGCACAGUGCCCUCUAUGAUGUCa -3' miRNA: 3'- -UCUACGUGUCACGGGAGAUACUACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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